摘要
目的探究单壁碳纳米管(single-walled carbon nanotubes,SWCNTs)对小鼠成神经瘤细胞(N2a)的细胞毒性作用。方法采用不同浓度的SWCNTs悬液(0、12.5、25、50μg·ml-1)对N2a细胞进行24 h染毒处理后,检测细胞活力,细胞活性氧(reactive oxygen species,ROS)、还原型谷胱甘肽(GSH)水平。结果随着N2a染毒浓度的增加,N2a细胞活力下降,ROS含量增加,GSH含量下降。在维生素C(Vit C)存在的情况下,最高浓度组(50μg·ml-1)产生的细胞损伤最大程度上得到缓解。结论 SWCNTs的暴露在某种程度上可对N2a细胞产生一定的细胞毒性,并且Vit C可以通过降低氧化损伤对N2a细胞起到一定的保护作用。
Objective To explore the cytotoxicity of N2a cells induced by the exposure of single-walled carbon nanotubes （SWCNTs）. Methods N2a cells were exposed to SWCNTs suspension at different concentrations （0,12. 5,25,50 ug. mL-1 ） for 24 h. MTT assay was used to analyze the viability of N2a cells. Additionally,reactive oxygen species （ROS） and glutathione （GSH） levels were measured to determine the oxidative stress. Results It was demonstrated that as the exposure concentration to SWNTs increased, the vitality of N2 a cells decreased, ROS content increased and GSH content decreased. In existence of Vit C, cell damage in the experimental group treated with SWNTs of 50 ug. mL-1 , the highest exposure concentration, was alleviated to the maximum extent. Conclusion All results suggested that exposure to SWCNTs could induce cytotoxicity in N2a cells to certain extent via oxidative damages. However, as antioxidant, Vit C could protect N2a cells by reducing the level of oxidative damage.
出处
《公共卫生与预防医学》
2014年第1期11-14,共4页
Journal of Public Health and Preventive Medicine
基金
国家自然科学基金青年基金项目(21103059)
