摘要
目的:探讨辣椒素剂量对去势雌性大鼠血脂、肝脂及盲肠内环境的影响。方法:选用30只雌性SD大鼠随机分组为5组,其中1组大鼠进行伪切除手术作对照(伪切除组),另外4组大鼠做双侧卵巢切除手术,基础饲料喂养恢复1周后,分为不灌胃辣椒素(空白组)和灌胃5.0、10.0、15.0 mg/(kg·d)辣椒素剂量组(低、中、高剂量组)。喂食相同基础饲料28 d后解剖,测定大鼠血清和肝脏中胆固醇、甘油三酯含量及盲肠面积,盲肠内容物水分含量、pH值、短链脂肪酸和微生物等指标。结果:空白组大鼠的体质量、采食量、血脂、肝脏总脂肪、总胆固醇、甘油三酯总含量均显著高于伪切除组。辣椒素可降低双侧卵巢切除大鼠的体质量增加量、血清和肝脏中胆固醇和甘油三酯含量,且具有明显的剂量关系;同时可以降低盲肠组织总质量、盲肠体质量比、盲肠内容物短链脂肪酸含量,显著降低盲肠内容物有益菌数量(P<0.05);辣椒素可以显著升高游离氨含量和pH值,增加有害菌数量(P<0.05),但辣椒素各剂量组之间没有显著性差异。结论:辣椒素对因雌激素缺乏引起高脂血症雌性大鼠具有较好的降血脂、降肝脂作用,但对盲肠内环境具有一定的损伤作用。
Objective： To investigate the effect of capsaicinoids on serum lipids, hepatic lipids and cecum environment in ovariectomized rats. Methods： Thirty mature female Wistar rats were divided into five groups. One group was shamovariectomized as sham reference group. The other four groups were double ovariectomized and assigned as model group and capsaicin groups at low（5.0 mg/（kg·d））, medium（10.0 mg/（kg·d）） and high doses（15.0 mg/（kg·d）） one week later. All rats were sacrificed after 28 days of feeding and analyzed for triglyceride（TG） and cholesterol（TC） in serum and liver, the area of cecum, the content of water, pH and short-chain fatty acid content in cecum, and intestinal microflora. Results： The body weight, feed intake, serum lipids, liver fat, hepatic total cholesterol, hepatic triglyceride in the control group were significantly higher than those in the sham group. Feeding capsaicinoids could reduce the body weight, body-weight gaining, plasma cholesterol and fat, total cholesterol and triglyceride in liver in a dose-dependent manner. In addition, capsaicinoids could also decrease the concentration of short-chain fatty acids and the amount of beneficial bacteria significantly（P ＆lt; 0.05）, and improve the concentration of free ammonia and pH of cecum content as well as the amount of harmful bacteria, significantly（P ＆lt; 0.05）, but there was no significant difference among different dose groups. Conclusion： Capsai cin may significantly decrease estrogen deficiency-induced hypercholesterolemia in rats, and damage the cecum environment.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第3期203-208,共6页
Food Science
基金
中央高校基本科研业务费专项(XDJK2009B004)
国家自然科学基金项目(2011GA31071529)
关键词
辣椒素
卵巢切除大鼠
血脂
肝脂
盲肠内环境
capsaicin
ovariectomized rat
serum lipids
hepatic lipids
cecum environment
