摘要
目的观察2种不同的培养方法对小鼠神经母细胞瘤细胞(N2a细胞)形态和功能的影响。方法取冻存的细胞经复苏后,分别用DMEM(Dulbecco’s modified Eagle’s medium)/Opti-MEM(1∶1)+5%胎牛血清(fetal bovine serum,FBS)与DMEM+10%FBS培养液进行培养,传至第7代时比较两组在细胞增殖率、细胞分化、粘附、迁移、自发凋亡方面的差异。结果 2种培养液均能用于培养小鼠神经母细胞瘤N2a细胞,DMEM+10%FBS组的细胞分化比例及粘附能力与DMEM/Opti-MEM(1∶1)+5%FBS组相比明显增高,细胞增殖率、迁移能力与细胞凋亡未见明显差异。结论 DMEM/Opti-MEM(1∶1)+5%FBS培养液可使小鼠神经母细胞瘤N2a细胞保持较低水平的分化率,因而更适合运用于诱导N2a分化的实验中。
Objective To investigate the effect of two different culture methods on the morphology and function of mouse neuroblastoma N2a cells. Methods Neuroblastoma N2a cells were resuscitated,and then cultured in DMEM（Dulbecco＇s modi- fied Eagle＇ s medium）/Opti-MEM（1 ： 1） ＋ 5 % fetal bovine serum（FBS） or DMEM＋ 10 % FBS. The differentiation, cell growth rate,adhesion,migration and spontaneous apoptosis were compared in the 7-passage cells cultured by the two different meth- ods. Results Neuroblastoma N2a cells could rapidly grow under both culture models. The differentiation rate and the adhesion ability of N2a cells in DMEM＋ 10 %FBS culture media were significantly greater than in DMEM/Opti-MEM（ 1 ： 1）＋ 5 % FBS media. No difference in the cell growth rate, migration and apoptosis was found in cells cultured in the two different media. Conclusion DMEM/Opti-MEM（1 ： 1） ＋ 5 % FBS is more suitable for N2a differentiation assay than DMEM＋ 10 % FBS due to the lower differentiation rate in DMEM/Opti-MEM（1 ： 1）＋5%FBS.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2014年第1期28-31,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.81371380)
关键词
N2A细胞
培养
分化
增殖
粘附
迁移
凋亡
N2a cells
culture
differentiation
proliferation
adhesion
migration
apoptosis
