摘要
目的 分析妊娠期肝内胆汁淤积症（ICP）孕妇产新生儿脐动脉血清A549细胞半胱氨酸蛋白酶-3（Caspase-3）的表达及凋亡,探讨胆汁酸引起新生儿肺损伤的相关机制.方法 以A549细胞为研究对象,将细胞随机分成正常对照组和ICP组.正常对照组采用正常培养基进行培养,ICP组从ICP孕妇胎儿娩出后在胎盘端收集脐动脉血并分离,按体积比1∶10配成ICP血清对A549细胞进行干预.24 h后检测A549细胞乳酸脱氢酶漏出率、Caspase-3表达和细胞凋亡率.结果 光镜下观察A549细胞Caspase-3的阳性细胞呈黄褐色表达于胞质中.ICP组细胞乳酸脱氢酶漏出率[（34.68±0.77）％]、Caspase-3表达的光密度值（981.77±55.21）、细胞凋亡率[（27.86±0.53）％]较正常对照组[（17.39±0.66）％、（540.63±38.41）、（6.99±0.11）％]明显增加,差异均有统计学意义（t=-45.70、-15.96、-134.41,P均＜0.05）.结论 ICP孕妇产新生儿脐动脉血清可诱导A549细胞Caspase-3表达增加,并使A549细胞凋亡明显增加,可能导致新生儿肺损伤.
Objective To explore the cause of bile acid-induced lung injury through investigating the cell apoptosis and the expressions of Capsase-3 in A549 cell of umbilical artery serum in neonates delivered by women with intrahepatic cholestasis of pregnancy.Methods A549 cell was used as target cell.The cultural cells in orifice were divided into control group and intrahepatic cholestasis of pregnancy-serum attacking group.The cells of control group were cultivated with normal nutritive medium.The umbilical arterial blood was cowered from the placental end of pregnant women with intrahepatic cholestasis after the baby had been delivered.Then the serum was gathered,and the cells of the intrahepatic cholestasis of pregnancy-serum attacking group were attacked by intrahepatic cholestasis of pregnancyserum.After 24 hours,lactate dehydrogenase leakage rate,expression of Caspase-3 and the apoptosis rate of the cells in the 2 groups were detected,respectively.Results The expression of Caspase-3 in A549 cells was observed in the light microscope,and Caspase-3 expre-ssion in the cytoplasm was brown.The lactate dehydrogenase leakage rate [（34.68 ±0.77） ％],the integrate optical density value （981.77 ± 55.21） of the expression of Caspase-3 and the rate of apoptosis [（27.86 ± 0.53） ％] of cells in intrahepatic cholestasis of pregnancy group were significantly higher than those of the cells in control group[（17.39±0.66）％,（540.63 ±38.41）,（6.99 ±0.11）％] （t =-45.70,-15.96,-134.41,all P ＜ 0.05）.Conclusions Umbilical artery serum in neonates delivered by women with intrahepatic cholestasis of pregnancy can induce apoptosis of A549 cells by up-regulating the expression of Caspase-3,and this was the potential machine of bile acid-induced lung injury in newborn infants.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第2期98-100,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
四川省卫生厅科研项目（090393）
四川省卫生厅科研基金资助项目（100411）
