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吐温-80对液氮冷冻处理的双歧杆菌的冻伤保护

Protection of Tween-80 against Liquid Nitrogen Frozen Damage of Bifidobacterium
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摘要 向双歧杆菌培养基中添加吐温-80,可增加双歧杆菌的细胞膜柔韧性和弹性。经液氮冷冻处理24h后,测定双歧杆菌细胞漏出液中乳酸脱氢酶的活力,结果表明添加吐温-80,可显著降低细胞培养液中的乳酸脱氢酶的漏出率(p<0.05),其中以添加0.025%吐温-80的效果尤为明显。 Adding tween-80 to bifidobacterium culture made the membrane of bifidobacterium more elastic and flexible. After frozen treated by liquid nitrogen for 24h, the result indicated that th6 leak of lactate dehydrogenase in the cell cultured medium decreased significantly, and adding 0.025% tween-80 was more distinct.
出处 《乳业科学与技术》 2006年第4期169-171,共3页 JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
关键词 双歧杆菌 吐温-80 乳酸脱氢酶 bifidobacterium Tween-80 lactate dehydrogenase
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