摘要
目的了解江西省2009~2011年〈5岁儿童轮状病毒（Rotavirus,RV）腹泻的流行病学特征,以及RV的血清型和基因型,为研发疫苗提供参考。方法采用酶联免疫吸附试验（Enzyme-linked Immunosorbent,ELISA）检测粪便标本中的RV抗原;对于抗原检测阳性的标本,用ELISA检测RV的G血清型,对于未确定型别的毒株再利用嵌套式（Nested）逆转录-聚合酶链反应（Reverse Transcription-Polymerase Chain Reaction,Nested-RT-PCR）鉴定型别,P分型直接采用RT-PCR方法。结果在江西省儿童医院,共采集〈5岁急性腹泻患儿的粪便标本624份,其中234份采自门诊患儿,390份采自住院患儿。共检出287份RV阳性粪便标本,总检出率为46.0%。10~12月是RV腹泻的高发季节。RV检测阳性血清型鉴定为G3型,是流行优势毒株;基因型鉴定P[8]型占73.3%。结论江西省〈5岁儿童RV腹泻全年均有发病,但以第四季度发病居多,发病人群以〈18月龄小儿为主;RV的血清型以G3型为主,基因型以P[8]型为主。
Abstract： Objective To understand the epidemiological characteristics among children who were 〈 5 years old in Jiangxi province and study on the serotype and genotype of rotavirus（RV） diarrhea, so as to provide basis for vaccine development. Methods Enzyme-linked immunosorbent assay （ELISA） was taken for detecting antigen of RV in stool samples; and ELISA was taken for detecting the G serotype of RV in positive samples, and the nested reverse transcription-polymerase chain reaction（Nested RT-PCR） was taken for detecting unidentified serotype; RT-PCR was taken for detecting the P genotype of RV. Results In Jiangxi provincial children＇s hospital, 624 samples were collected from the children who were 〈 5 years old with acute viral diarrhea, among them, 234 samples was from outpatient children and 390 samples was from hospitalized children. 287 samples were positive and the total positive rate was 46. 0% （287/624）. The major epidemic season of rotavirus diarrhea was during October and December. The major G serotype of RV in positive samples for antigen detection was G3, and the genotype P8 accounted for 73.3%. Conclusion There were rotavirus diarrhea cases among children who were 〈5 years old in four seasons in Jiangxi province, and the fourth quarter was the peak season. Most of the cases were children who were 〈 18 Months old. The major serotype of rotavirus diarrhea was G3, and the major genotype was P8.
出处
《中国疫苗和免疫》
CAS
2014年第1期59-61,共3页
Chinese Journal of Vaccines and Immunization
基金
江西省卫生厅科学技术计划项目(编号:20093141)
关键词
〈5岁儿童
轮状病毒
腹泻
流行病学特征
分子流行病学
〈 5 years old children
Rotavirus
Diarrhea
Epidemiological characteristics
Molecular epidemiology
