摘要
目的通过对中国2010年脊髓灰质炎(脊灰)实验室网络(Polio Laboratory Network,PLN)监测数据(未包括香港、澳门特别行政区和台湾地区,下同)的分析,对其运转情况进行评估,为中国维持无脊灰提供实验室依据。方法对中国免疫规划监测信息管理系统数据库中,31个省(自治区、直辖市,下同)报告的急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例个案调查表和中国疾病预防控制中心(Center for Disease Control and Prevention,CDC)病毒病预防控制所国家脊灰实验室(National Polio Laboratory,NPL)的监测数据库进行统计分析,对PLN的各项运转指标进行评价。结果中国2010年PLN收集到5273例AFP病例的10 427份粪便标本,14天内双份粪便标本采集率为91.6%,合格粪便标本采集率为91.4%。按照世界卫生组织(World Health Organization,WHO)2004年修订的《脊灰实验室手册》第4版的操作规范,进行病毒分离和脊灰病毒(Poliovirus,PV)阳性毒株的血清学鉴定,鉴定结果 28天内反馈率96.6%。2010年,在5230例AFP病例的137例粪便标本中分离到PV,分离率为2.61%;677例粪便标本中分离到非脊灰肠道病毒,分离率12.95%。2010年,NPL收到PLN送检的137份PV分离物,按单血清型共计353株。用VP1编码区核苷酸序列测定与分析的方法,对所有PV进行了型内鉴定,发现4株疫苗衍生脊灰病毒,未发现脊灰野病毒。2010年,NPL和31个省级CDC脊灰实验室都通过了WHO盲样标本考核,其中12个接受并通过了WHO现场评估认证。结论中国PLN 2010年运转正常,为维持无脊灰提供了实验室依据。
Objective In order to evaluate the running status and provide the laboratory data for maintaining polio-free status in China, the virology surveillance database of Chinese polio laboratories network （PLN, not include Hong Kong Special Administrative Region, Macao Special Administrative Region, Taiwan sheng） in 2010 were analyzed. Methods The case investigation data of acute flaccid paralysis （AFP） cases reported by 31 provinces （ municipalities, antonomous regions） through expanded programme on immunization surveillance information management system and the database of national polio laboratory （NPL） were analyzed, and the indicators of running status of Chinese PLN were evaluated. Results 10,427 stool samples were collected from 5,273 AFP cases by Chinese PLN in 2010, collection rate of double stool within 14 days was 91.6%, collection rate of qualified stool was 91.4%. According to 4th World Health Organization （WHO） polio laboratory manual, the rate of viral isolation and identification performed within 28d was 96.6%. There are 137 polioviruses （PV） and 677 were isolated form AFP cases, the isolation rates were 2.61% and 12.95% respectively. 137 polio positive isolates were sent to NPL by 31 provincial polio laboratories. Protocol for intratypic differentiation in NPL was VP1 sequencing. There were 137 single serotype of poliovirus were performed VP1 sequencing, 4 vaccine- derived polioviruses were detected iH 2010 and no wild PV was found. All polio laboratories passed the proficiency test with the same panl as NPL, 12 provincial polio laboratories joined and got full accreditation for on-site review by all experts between 2009 and 2010. Conclusion The running status of Chinese PLN was good, polio-fr~＇e status was maintained in China in 2010; the Chinese PLN ran normally, the laboratory surveillance system was sensitive and laboratory data were provided reference for maintaining the polio-free status in China.
出处
《中国疫苗和免疫》
CAS
2014年第1期45-50,共6页
Chinese Journal of Vaccines and Immunization
基金
行使世界卫生组织西太平洋区脊髓灰质炎参比实验室职能,2010年卫生部疾病控制专项
关键词
脊灰实验室网络
运转
评价
Poliomyelitis laboratories network
Running
Evaluation
