摘要
目的对2015—2019年河南省急性弛缓性麻痹(acute flaccid paralysis,AFP)病例中,在转人类脊髓灰质炎病毒受体小鼠肺细胞(mouse cell line expressing the gene for human cellular receptor for poliovirus,L20B)增殖的非脊髓灰质炎肠道病毒基因定型和序列特征分析。方法利用VP4-VP2编码区引物EVP4和OL68-1进行肠道病毒鉴定,均为柯萨奇A组病毒(coxsackievirus A,CV-A),分别为CV-A4、CV-A8和CV-A10。在利用CV-A4、CV-A8和CV-A10 VP1区特异性引物进行VP1区序列扩增和亲缘进化树构建。结果9例L20B阳性分离物中,1例为CV-A4,1例为CV-A8,7例为CV-A10。通过亲缘进化树构建,CV-A4属于D基因型,CV-A8属于B基因型,CV-A10属于E基因型。结论河南省L20B细胞增殖的非脊髓灰质炎肠道病毒为CV-A4、CV-A8和CV-A10,通过这些病毒基因特征分析对手足口病和AFP预防控制具有重要意义。
Objective To isolate non-polio enteroviruses from patients with acute flaccid paralysis(AFP)from 2015 to 2019 in Henan Province,and to identify and genetically characterize the isolates proliferated on L20 B cells(mouse cell line expressing the gene for human cellular receptor for poliovirus).Methods The primers used for EVP4 and OL68-1 which were based on VP4-VP2 gene located in the coding region,were designed to isolate from coxsackievirus A(CV-A),including CV-A4,CV-A8 and CV-A10.Full-length VP1 genes of the three isolates were amplified by RT-PCR.The VP1 gene homology was analyzed by Megalign software.Results In the experiment,9 isolates were identified,including one case of CV-A4,one case of CV-A8,and 7 cases of CV-A10.Phylogenetic tree analysis indicated that CV-A4 belonged to D genotype,CV-A8 belonged to B genotype,and CV-A10 belonged to E genotype.Conclusion The CV-A viruses multiplied on L20 B cells,and caused cytopathic effect.The genetic characteristics showed that CV-A4,CV-A8 and CV-A10 were predominant CV-A viruses identified in Henan Province,these viruses are of great significance in the prevention and control of hand,foot and mouth disease and AFP.
作者
张璐
肖金波
王文慧
吕宛玉
徐瑾
杨建辉
ZHANG Lu;XIAO Jin-bo;WANG Wen-hui;Lv Wan-yu;XU Jin;YANG Jian-hui(Institute of EPI Laboratory,Henan Center for Disease Control and Prevention,Zhengzhou 450016,Henan Province,China;不详)
出处
《微生物学免疫学进展》
CAS
2020年第6期30-34,共5页
Progress In Microbiology and Immunology
基金
河南省医学科技攻关计划省部共建青年项目(SB201904014)
关键词
柯萨奇A组病毒
急性弛缓性麻痹
手足口病
Coxsackie virus A
Acute flaccid paralysis(AFP)
Hand,foot and mouth disease
