摘要
目的 为研究角蛋白克隆抗体免疫组化法在检测淋巴结微转移灶上的意义。方法 采用三种角蛋白单克隆抗体AE1,AE3、AE1/AE3,对本院 4 7例大肠癌术后患者 331枚淋巴结用免疫组化法重新检查 ,结果 36枚淋巴结存在微转移灶 ( 11 9% ) ,Duke’sC期患者微转移灶检出率高于Duke’B期患者 (P <0 0 5)。结果 随访显示有微转移灶Duke’sB期病人肿瘤复发 ,远处扩散转移均较无微转移病人高 (P <0 0 5)。
Objective:To study the significance of detection of lymph node micrometastasis by immunohistochemical method with monoclonal anti-karatin antibody in patients with colorectal cancer.Methods:We examined 331 lymph nodes obtained from 47 colorectal cancer patients who underwent a curative operation and diagosed by using immunohistochemistry SB techniques.Monoclonal mouse anti-keratin antibodies(AE1,AE1/AE3,AE3)were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Results:A total of 36 micrometastasis among 331 lymph nodes(11 9%) were found.Duke's C staging patients had more lymph nodes metastasis than that in Duke's B staging patients(P<0 05).Postoperative follow-up revealed that recurrence rate was high and the latent period of distant metastasis was much shorter in patients with occult mirometastasis than those without.Conclusions:Even in histologically negative lymph nodes,there is a considerable possibility that micrometastasis may exist.The immunohistochemical detection of monoclonal anti-keratin antibody can explicate clinical staging and as an indication for colorectal cancer therapy.
出处
《中国医师杂志》
CAS
2001年第1期13-15,共3页
Journal of Chinese Physician
关键词
大肠癌
淋巴结
微转移
角蛋白
免疫组织化学
Colorectal
Cancer,Keratin
Node
Micrometastasis
Immunohistochemistry
