摘要
采用高浓度的胰岛素诱导HepG2细胞出现葡萄糖代谢异常,从胰岛素的添加量和作用时间角度探讨建立胰岛素抵抗细胞(HepG2/IR)模型的最佳条件;分别设立阴性对照组、胰岛素抵抗模型组、盐酸吡格列酮阳性对照组以及苦瓜皂苷干预组(100、300、500、700μg/mL),利用葡萄糖测定试剂盒检测各组葡萄糖消耗量,同时MTT法评价苦瓜皂苷对HepG2/IR细胞活性的影响。结果表明,30μg/mL胰岛素诱导处理HepG2细胞36h是产生胰岛素抵抗的最适条件。与HepG2/IR模型组相比,300μg/mL的苦瓜皂苷不仅可以显著改善HepG2/IR细胞的葡萄糖消耗量,还能提高其细胞活性。
High concentration insulin was added into HepG2 cells to induce glucose metabolism abnormaLThe optimal conditions of insulin resistant HepG2 cells（HepG2/IR）model was investigated from insulin dose and effect time.HepG2 cells were divided into control group, insulin resistant group, pioglitazone hydrochloride group, bitter melon saponin groups （100,300,500,700ug/mL） .The glucose consumption was measured by glucose oxidase method（GOD）.The cell activity was estimated by M-IT method.These results showed that treatment with 30μg/mL insulin for 36h was the best condition for induction of HepG2/IR model.Compared with HepG2/IR group,treatment with 300μg/mL bitter melon saponin not only could alleviate insulin resistant HepG2 cells significantly, but cell activity was increased.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第5期338-340,345,共4页
Science and Technology of Food Industry
基金
天津市科技支撑计划项目(13ZCZDNC01800)
天津农学院科学研究发展基金计划(2011N03)
