摘要
目的 探讨高温对人前列腺癌细胞株PC - 3m和LNCaP生长的影响。方法 用成集落实验和细胞生长曲线分析癌细胞在 43℃和 37℃作用 1h或 2h后的生长抑制率。结果 PC - 3m在 43℃作用 1h和 2h的平均集落形成抑制率分别为(30 .8± 2 .4) %和 (5 6 .6± 14.7) % ;LNCaP分别为 (4 1.3± 12 .6 ) %和 (94.2± 6 .3) % ,差异比较均具有极显著性意义 (分别为P <0 .0 0 5和P <0 .0 0 1)。与细胞生长曲线测定结果一致。结论 高温对人前列腺癌细胞株具有细胞毒性作用 ,温度和作用时间对癌细胞生长的抑制也有重要影响 ;热疗作为治疗前列腺癌的一种辅助疗法 。
Objective To study the effect of hyperthermia on growth of human prostatic cancer cell lines PC-3m and LNCaP in vitro.Methods PC-3m and LNCaP were exposed to high temperature at 43 ℃ or 37 ℃ for different duration(1 h or 2 h),and cell survival inhibition rate was evaluated by colony formation assay and measurement of cell growth rate. Results When PC-3m and LNCaP were exposed to 43 ℃ for 1 or 2 hours,their mean inhibition rates of colony formation were (30.8±2.4)% and (56 6±14 7)%,and (41.3±12.6)% and (94.2±6.3)%,respectively.There was high significant difference between both cell lines( P<0.005 and P <0.001 respectively).These were similar to the results obtained in the cell growth curves. Conclusion Results indicate that induced hyperthermia has a cytotoxic effect on human prostatic cancer cells,moreover,temperature and exposure time have an important impact on the cell inhibition rate.Hyperthermia as a potential useful adjunctive therapy for local control of prostatic cancer merits further investigation.
出处
《山西医科大学学报》
CAS
2001年第1期7-9,共3页
Journal of Shanxi Medical University
