摘要
[目的]研究温经通痹汤对坐骨神经损伤大鼠痛阈和脊髓背角NR2B表达的影响。[方法]建立坐骨神经损伤大鼠模型,将100只大鼠随机分为模型组(50只)和温经通痹汤组(50只),在造模前及造模后第1、3、7、15、30d时,测定两组大鼠的缩腿阈(Paw Withdrawal Thresholds,PWTs)作为痛阈。造模后对温经通痹汤组大鼠采用温经通痹汤灌胃2次/d、10mL·kg-1/次治疗,在造模后第1、3、7、15、30d等时间点各处死大鼠10只,取腰段脊髓做脊髓背角NR2B受体测定,并进行分析。[结果]两组大鼠造模后第1d的PWTs明显低于造模前的PWTs值(P<0.01),显示造模成功。模型组大鼠造模后第7、15、30d的PWTs值分别为16.28±0.49g、18.21±0.38g和20.05±0.59g,明显高于造模后第1d的13.97±0.44g,差异有统计学意义(P<0.05,P<0.01)。温经通痹汤组大鼠造模后第7、15、30d的PWTs值分别为18.64±0.98g、22.32±1.61g、19.94±0.40g,均高于造模后第1d的13.69±0.58g,差异有统计学意义(P<0.01)。两组间比较,造模后第7、15d时,温经通痹汤组大鼠PWTs值均高于模型组大鼠的PWTs值,差异有统计学意义(P<0.05)。与造模后1d时比较,模型大鼠脊髓背角NR2B的亚基表达在术后第3、7、15、30d差异有统计学意义(P<0.01);温经通痹汤组脊髓背角NR2B的亚基表达只有在造模后第7、15、30d有非常显著性差异(P<0.01)。两组组间比较,温经通痹汤组脊髓背角NR2B的表达在3、7、15 d显著低于对照组,差异有统计学意义(P<0.01),提示温经通痹汤治疗对CCI模型大鼠脊髓背角的NR2B亚基表达有抑制作用。[结论]温经通痹汤能有效提高坐骨神经损伤大鼠(CCI模型)的痛阈,并在一定治疗时间内存在镇痛累积效应。温经通痹汤在早期较好地抑制脊髓背角NR2B的表达,与改善痛阈的时点吻合,初步说明温经通痹汤提高痛阈与调节脊髓背角NR2B亚基的表达有关。
[Objective] To study the effect of Wen Jing Tong Bi Decoction on pain threshold of sciatica and expression of NR2B in spinal dorsal horn. [Methods] 100 Spraque-Dawley rats were randomly divided into 2 groups： Model group（n=50） and Wen Jing Tong Bi Decoction group（n=50）. The left sciatic nerve of rat was isolated and constricted. After operation, the Wen Jing Tong Bi Decoction group was treated with 10ml·kg-1 twice a day; and the Model group was administered with the same dose of saline. Paw withdrawal thresholds（PWTs） are used as the pain threshold. Al rats were observed PWTs and executed at the pre-operation and the 1st, 3rd, 5th, 7th, 15th, 30th day after operation. The lumbar spinal cord was used to detect the expres-sion of NR2B receptor in spinal dorsal horn.[Results] 1.Post-operation 1 day, the PWTs of rats decreased obviously. So the model of scratic nerve was made successful y. 2.In model group, the PWTs of postoperative 7th, 15th, 30th day were much higher than those in postoperative 1st day（16.28 ±0.49g vs 13.97±0.44g, 18.21±0.38g vs 13.97±0.44g, 20.25±0.59g vs 13.97±0.44g, P〈0.05 or P〈0.01）. It was suggested that the pain releved since operative 7th day. 3.In Wen Jing Tong Bi Decoction group, the PWTs of operative 7th, 15th, 30th day were much higher than those in postoperative 1st day（18.64 ± 0.98g vs 13.69±0.58g, 22.32±1.61g vs 13.69 ±0.58g, 19.94±0.40g vs 13.69±0.58g, P〈0.01）, and there were significant differences. 4. The PWTs of op-erative 7th and 15th day in Wen Jing Tong Bi Decoction group were much higher than those in model group（ P〈0.05）.There was no difference in the pain threshold of operative 30th days between two groups（ P〉0.05）, and the pain threshold in postoperative 30 days had no significant difference compared with that in preoperative（P〉0.05）. 5.The result of NR2B in spinal dorsal horn： there were significant differences in the actual staining gray scale at any time of post-operation. In Wen Jing Tong Bi Decoction group, t
出处
《浙江中医药大学学报》
CAS
2014年第1期17-21,共5页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药管理局项目(2007CA035)~~
关键词
温经通痹汤
坐骨神经损伤
痛阈
NR2B
Wen Jing Tong Bi Decoction
sciatic nerve injury
pain threshold
NR2B
