摘要
目的观察大鼠皮肤急性深Ⅱ度β射线损伤创面自然愈合过程中β_1整合素表达阳性细胞的动态变化,探讨急性β射线皮肤损伤创面的难愈机制。方法将96只SD大鼠随机分为实验组(48只)与对照组(48只),实验组建立大鼠皮肤急性深Ⅱ度β射线损伤创面模型;对照组建立大鼠皮肤深Ⅱ度热力烫伤创面模型。分别于伤后1 d、3 d、7 d、14 d、21d、28 d、35 d记录创面变化,并取创面组织标本,行HE染色和免疫组化染色,观察创面愈合过程中的组织变化和β_1整合素阳性细胞的变化。结果实验组大鼠创面愈合时间较对照组长,经统计学分析,差异有统计学意义(P<0.01);通过对β_1整合素的检测发现,照射及烫伤后1 d、3 d、7 d、14 d、21 d的β_1整合素阳性细胞IOD值比较,差异有统计学意义(P<0.05),对照射及烫伤后28 d、35d的β_1整合素阳性细胞IOD值进行比较,差异无统计学意义(P>0.05)。结论皮肤急性深Ⅱ度β射线损伤创面愈合时间相对缓慢,且在愈合过程中β_1整合素阳性细胞增殖分化延迟。
Objective To investigate the healing mechanisms of refractory acute β-ray-injured skin wotmd by observing the dynamic changes of cells expressing integrin β1 during the natural healing process of acute β-ray-injured deep second degree skin wound in rats. Methods 96 SD rats were randomly divided into an experimental group （ n = 48） and a control group （n =48）. Build a rat model of acute deep second-degree skin wound caused by β-ray in the experimental group ; set up a rat model of deep second-degree skin wound of thermal scald in the control group. On 1 d, 3 d, 7 d, 14 d, 21 d, 28 d, 35 d post-injury＇, record the wound changes and take wound tissue sample for HE staining and innnunohistochemical staining to observe the histological changes and changes of ceils expressing integrin I^l during the process of wound healing. Results Compared with the control group, the wound healing time of rats in the experimental group was longer and the difference was significant according to the statistical analysis （P 〈 0. 01 ）. The detection of integrin β1 showed there was a statistically significant difference between the two groups in terms of IOD value of cells expressing integrin β1 on day 1, day 3, day 7, day 14, day 21 post-injury （P 〈0. 05）, but the IOD value on day 28 and day 35 showed no statistically significant difference between the two groups （P 〉 0. 05）. Conclusion The healing time of acute deep-second degree skin wound caused by B-ray was relatively longer and the proliferation and differentiation of cells expressing integrin B1 were delayed during the healing process which could be one of the reasons why the healing of B-ray-injured acute deep-second degree skin wounds was delayed.
出处
《中国烧伤创疡杂志》
2014年第1期30-35,共6页
The Chinese Journal of Burns Wounds & Surface Ulcers
