摘要
以新疆吐鲁番的赤霞珠葡萄籽为研究对象,利用传统溶剂法、超声波辅助法、微波辅助法和超声微波双辅助法提取其中的原花青素,经过AB-8大孔树脂初步纯化后进行抗菌抗氧化试验。分别考察了原花青素的总抗氧化能力、对·OH、O2-·、DPPH·清除作用及其对肠道致病菌的抑制作用。结果表明:原花青素对·OH、O2-·、DPPH·的最大清除率分别为65.71%、64.57%和87.55%。其对革兰阳性菌较革兰阴性菌的抑制作用强且原花青素对细菌生长的抑制作用与其浓度成正比。
In this paper, grape seeds of Cabernet Sauvignon from Turpan Xinjiang was taken as study object. Four extraction treatments（solvent extraction, ultrasonic assisted extraction, microwave extraction and ultrasonic treatment followed by microwave extraction） were used to extract the proanthocyanidins. The antibiotic and antioxidant test were used after purified by AB-8 macroporous resin. The total antioxidant capacity of procyanidins, its scavenging effect on ·OH, O 2-·, DPPH·, and its inhibitory effect on pathogenic bacteria of intestinal tract were investigated and the results showed that the maximum removal of proanthocyanidins on ·OH, O 2-·, DPPH· was 65.71%, 64.57% and 87.55%. It was higher in inhibitoryeffect of proanthocyanidin against Gram-positive bacteria than Gram-negative bacteria. Proanthocyanidin inhibition on bacteria growth was in direct proportion to its concentration.
出处
《食品科技》
CAS
北大核心
2014年第2期209-215,共7页
Food Science and Technology
基金
国家"十二五"农村领域国家科技计划课题(2011BAD27B00)
关键词
赤霞珠葡萄籽
原花青素
抗氧化
抑菌作用
grape seeds of Cabernet Sauvignon
proanthocyanidins
antioxidant
bacteriostasis
