摘要
根据猪链球菌 2型的mrp基因自行设计和合成了一对可扩增长度为 885bp目的片段的引物 ,成功地建立了检测溶菌酶释放蛋白 (MRP)的PCR方法。用XbaⅠ内切酶进行酶切 ,获得了与预期一致的 5 78bp和 30 7bp的 2个片段。并进行了PCR的特异性试验和敏感性试验。对马链球菌兽疫亚种、猪丹毒杆菌、猪肺疫巴氏杆菌和猪肺炎支原体的PCR检测结果均呈阴性 ;检测的敏感度可达 10 0个细菌。另外 ,对 9株从病猪体内分离的猪链球菌 2型菌株进行了检测 ,8株呈阳性 ;对 15份正常屠宰猪扁桃体分离物的检测结果是 1份为阳性。结果表明此法特异性和敏感性均很高 ,可作为MRP快速诊断和流行病学调查的手段。
A PCR assay for the rapid and sensitive detection of MRP of Streptococcus suis type 2 was developed.The PCR primers based on the MRP gene of S.suis type 2 could extend a 885bp PCR product.With Xba I digestion,the PCR product could produce a 578bp and a 307bp DNA fragment as expected.Using the same pairs no PCR product was detected from S.equisimilis,E.rhusiopathiae,P.pneumotropica, and M.hyopneumoniae.The PCR could detect 100 bacteria.Of 9 strains of S. suis type 2 isolated from diseased pigs,8 were positive.Of 15 porcine tonsillar specimens,1 was positive.The results demonstrated that the PCR is a highly specific and sensitive diagnostic tool for the detection of MRP from S.suis type 2 and can be used for fast diagnosis and epidemic survey.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第1期20-22,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"973"项目资助
