摘要
以茄蒂为原料,研究了酶解超声协同提取茄蒂多糖条件及抗氧化能力。在pH5、50℃水浴温度下用1%纤维素酶酶解30min,采用响应面法优化超声提取茄蒂多糖条件。通过测定对羟自由基、超氧自由基清除能力和总还原力,评价茄蒂多糖抗氧化活性。结果表明,茄蒂多糖最佳提取条件为:料液比1∶36(g/mL),超声时间22min,超声温度68℃。在此条件下多糖得率为5.49%。茄蒂多糖对羟自由基、超氧自由基清除能力和还原力均表现较好的效果,其清除羟自由基、超氧自由基的IC50值分别为0.12、0.22mg/mL。茄蒂多糖抗氧化性略低于VC。
Using enzymatic ultrasonic assisted that investigated for extraction of polysaccharide from Solanum melongena sepa, and their antioxidant activity were determined. The Solanum melongena sepal were hydrolyzed in pH5. 1 % cellulase, reaction temperature 50℃ for a hydrolysis duration of 30min. The extracting condition was optimized using response surface analysis. The antioxidant properties of polysaccharide were studied by scavenging of hydroxyl free radical, superoxide anion free radical and reducing power. Results showed that optimal extraction conditions were determined to be 1 ：36（g/mL） ,22min and 68OC. Under these conditions, the extraction rate of polysaccharides was 5.49%. The polysaccharides had high abilities to scavenge hydroxyl and superoxide anion free radicals and excellent reducing power. The corresponding IC50 values against hydroxyl and superoxide anion free radicals were 0.12mg/mL and O.22mg/mL, however, had weaker antioxidant activity than Vc.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第4期246-250,255,共6页
Science and Technology of Food Industry
基金
甘肃省高等学校研究生导师科研项目(1009-08)
甘肃省高校河西走廊特色资源利用省级重点实验室面上项目(XZ1008)
关键词
茄蒂
多糖
超声酶解协同
响应面法
抗氧化性
Solanum melongena sepal
polysaccharides
enzymatic hydrolysis ultrasonic
response surface methodology
antioxidantion
