摘要
目的研究细胞因子诱导的杀伤细胞(CIK)联合顺铂对人肺腺癌细胞系A549的杀伤作用。方法在体外将健康者外周血单个核细胞(PBMC)用细胞因子诱导形成具有杀伤活性的CIK细胞。CIK、顺铂及CIK联合顺铂分别作用A549细胞24、48 h,MTT法检测细胞吸光度(A)值并计算各组细胞杀伤率。完全培养液培养CIK 48 h后,取上清液,按1∶1稀释后与A549细胞共培养24 h,用流式细胞仪检测A549细胞凋亡率。结果 CIK、顺铂对A549的杀伤活性呈时间、剂量依赖性(P<0.05)。5μg/mL顺铂联合效靶比为10∶1、20∶1的CIK共同作用A549细胞24 h,A549的杀伤率(%)分别为53.80±6.81,61.17±3.82,与单用顺铂及CIK相比,其对A549的杀伤率显著升高(P<0.05)。CIK上清液可诱导A549细胞凋亡。结论 CIK可增强顺铂对A549细胞系的杀伤作用。
Objective To investigate the lethal effect of cytokine-induced killer cells （CIK） combined with cisplatin on the human lung adenocarcinoma cell line A549. Methods The peripheral blood mononuclear cells （PBMC） from healthy persons were induced to be the CIK cells with cytotoxicity by cytokines in vitro. Then, A549 cells were incubated with CIK, eisplatin and CIK combined with cisplatin for 24 and 48 hours, respectively. The kill ratios of A549 cells were detected by the MTT assay. After CIK cells were incuba- ted with complete culture medium for 48 h, the supernatant was collected and diluted 1 ： 1 with medium, and then incubated with A549 cells for 24 h. Next, the apoptosis rate of A549 cells was detected by flow cytometry. Results Both CIK and cisplatin killed A549 cells in a dose- and time-dependent manner （ P 〈 0.05 ）. After A549 cells were incubated with 5 μg/mL of cisplatin combined with CIK with 10：1 and 20：1 of effector-target ratios for 24 h, the kill ratios of A549 cells were （53.80 ± 6.81 ）% and （61. 17± 3.82） %, respectively, which were significantly higher than that of cisplatin or CIK alone （ P 〈 0.05 ）. The supernatant of CIK cells could induce the apoptosis of A549 cells. Conclusion CIK may enhance the lethal effect of cisplatin on the lung adenoearcinoma cell line A549 in vitro.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2013年第12期915-918,共4页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金(81171653
30972703)
江苏省自然科学基金(BK2011246
BK2011247)
关键词
肺肿瘤
肺腺癌细胞株
细胞因子诱导的杀伤细胞
顺铂
lung cancer
lung adenocarcinoma cell line
cytokine-induced killer cell
cisplatin
