摘要
目的:优选远志总皂苷提取液的澄清工艺。方法:以固形物去除率和细叶远志皂苷保留率为评价指标,在单因素试验基础上,通过正交试验考察药液质量浓度、加入温度、搅拌速度及澄清剂加入量对远志总皂苷提取液澄清工艺的影响。采用HPLC测定细叶远志皂苷含量,流动相甲醇-0.05%磷酸溶液(65∶35),检测波长210 nm。结果:最佳澄清工艺为提取液浓缩至0.125 g·mL-1,保温搅拌加入B组分,用量0.8 g·L-1,间隔2 h后保温搅拌加入A组分,用量0.4 g·L-1,两组分水浴温度80℃,保温时间20 min,搅拌速度90 r·min-1,搅拌时间10 min;细叶远志皂苷保留率84.56%,固形物去除率21.97%。结论:ZTC1+1-Ⅱ型澄清剂用于澄清远志总皂苷提取液的效果良好。
Objective ： To optimize clarification process of extracts of total saponins from Polygalae Radixwith ZTC1 ＋ 1-Ⅱ clarifier. Method ： With solids removal rate and retention rate of tenuifolin as indexes, based on single factor tests, orthogonal test was adopted to optimize clarification process of extracts of total saponins from Polygalae Radix by taking the concentration of extracts, reaction temperature, stirring speed and dosage of ZTC1 ＋ 1-Ⅱclarifier as factors. HPLC was adopted to determine the content of tenuifolin with mobile phase of methanol- 0.05% phosphoric acid （65： 35） and detection wavelength of 210 nm. Result： Optimal clarification process was concentrated extracts to 0. 125 g .mL-1 , added component B with dosage of 0.8 g .L-1 by insulation stirring, then added component A with dosage of 0.4 g .L-1 after two hours, bath temperature of two components were 80 ℃ , stirring speeds were 90 r .min-1 , insulation time and stirring time were 20 and 10 min, respectively; Retention rate of tenuifolin and solids removal rate were 84.56% and 21.97%, respectively. Conclusion： ZTC1 ＋ 1- Ⅱnatural clarify agents had good clarification effect on extracts of total saponins from Polygalae Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第4期12-15,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2013ZX09103002-015)
关键词
远志
总皂苷
ZTC澄清剂
正交试验
单因素试验
细叶远志皂苷
Polygalae Radix
total saponins
ZTC1 ＋ 1- Ⅱ clarifier
orthogonal test
single factor test
tenuifolin
