摘要
目的：建立生脉超微粉中3种人参皂苷的含量测定方法。方法：采用kromasil C18（250 mm＆#215;4.6 mm，5μm）色谱柱，以乙腈（A）-水（B）为流动相，梯度洗脱0-35 min，19%A；35-55 min，19%-29% A；55-70 min，29%A；70-100 min，29%-40%A,流速1 mL＆#183;min-1，检测波长203 nm，柱温30℃，进样量10μL。结果：人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1线性范围分别为0.083-0.834μg、0.086-0.863μg、0.091-0.911μg；平均加样回收率（n=6）分别为100.7%、100.5%、100.5%。结论：本方法快速、灵敏，重现性好，适合于生脉超微粉中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的含量测定。
This study was aimed to establish an HPLC method to determine three ginsenosides in Shengmai ultra-micro powder. The kromasil C18 （250 mm ＆#237; 4.6 mm, 5 μm） was used as analytical column. The mobile phase was composed of acetonitrile （A） and water （B） with gradient elution （0-35 min, 19% A; 35-55 min, 19%-29% A; 55-70 min, 29% A; 70-100 min, 29%-40% A） at a flow rate of 1 mL＆#183;min-1. The detection wavelength was 203 nm and the column temperature was 30℃. The injection volume was 10 μL. The results showed that the linear ranges of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 were 0.083-0.834 μg, 0.086-0.863 μg, 0.091-0.911 μg, respec-tively. The average recovery rates （n = 6） were 100.7%, 100.5%, 100.5%, respectively. It was concluded that this method was quick, sensitive, repeatable and suitable to determine contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Shengmai ultra-micro powder.
出处
《世界科学技术-中医药现代化》
北大核心
2013年第8期1801-1804,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部国家重点基础研究发展计划(973计划)项目(2010CB735604):新药研制过程化学机理--中药制药过程控制技术模式和方式研究
负责人:萧伟
