摘要
目的:对不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1三种主要药效成分进行高效液相色谱的含量测定。方法:采用C18柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速为1.1 ml/min,检测波长为203 nm,柱温为30℃。结果:人参皂苷Rg1、Re、Rb1、在测定范围内有良好的线性关系,其平均回收率为97.2%～99.0%,RSD为1.61%～2.03%。结论:该方法准确可靠,重现性好,应用性强。
Objective：To detect ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 of three active principles in American ginseng from different regions by HPLC.Methods：A C18 column was used by gradient elution of acetonitrile-0.1% H3PO4 solution as the mobile phase,the flow rate was 1.1 ml/min and the detective wavelength was set at 203 nm,the column temperature was set at 30℃.Results：All of the three compounds showed good linearities and recoveries were in the range of 97.2%-99.0% with RSD of 1.61%-2.03%.Conclusion：The method is accurate and suitable for the determination of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1.
出处
《中国医药导报》
CAS
2011年第20期103-105,共3页
China Medical Herald
