摘要
本文考察了单宁酸对胰α-淀粉酶(PPA)的抑制作用,并通过紫外和荧光光谱法研究了二者的作用特性。研究发现单宁酸可明显抑制胰α-淀粉酶的活性,其作用类型为非竞争性抑制。单宁酸与胰α-淀粉酶的结合能自发进行,作用位点靠近PPA的色氨酸残基区域。二者结合后,可导致PPA在270nm附近的紫外吸收发生明显改变,并产生红移,同时可引起PPA荧光猝灭。
The inhibitory effect on porcine pancreatic cc-amylase（PPA）and its inhibitory mechanism of tannic acid （TA） was investigated by UV-vis absorption and fluorescent spectrometry.As a non-competitive inhibitor,tannic acid showed significant inhibitory effect on PPA.The TA-PPA interaction was a spontaneous molecular process, and the TA-PPA binding site was closer to PPA trytophan residues. The UV absorbance at 270nm of PPA decreased and the absorption bands showed red-shift when it interacted with tannic acid.In addition,the intrinsic fluorescence of PPA could be significantly quenched by tannic acid.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第3期63-66,共4页
Science and Technology of Food Industry
基金
国家自然科学基金(NSFC-31171656)
国家科技支撑计划(2012BAD37B09)资助
关键词
单宁酸
胰α-淀粉酶
抑制作用
紫外差谱
荧光光谱
tannic acid
pancreatic a-amylase
inhibition UV differential spectra turbidity
fluorescent spectra
