摘要
以活力、大小一致的菖蒲小植株为试材，以不添加铬的营养液为对照（CK），研究了5、10、20、40、60mg／L铬（VI）胁迫对菖蒲（Acorus calamus）根系活力（TTC）、叶绿素含量、维生素C含量、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性等生理生化特性的影响，为其作为水体环境修复材料的可行性提供科学参考。结果表明：菖蒲在低、中浓度（5-20mg／L）铬（Ⅵ）胁迫时叶绿素含量有所增加，而在高浓度（40、60mg／L）时显著下降；随铬（Ⅵ）胁迫浓度的增加，可溶性蛋白质含量总体上显著上升，可溶性糖含量总体上呈下降趋势，脯氨酸含量先升后降，而维生素C含量在低浓度时降幅较小，在高浓度时降幅较大。随着铬（Ⅵ）处理浓度的增加，菖蒲根系活力、还原型谷光甘肽（GSH）含量、MDA含量均为先增后降，而超氧阴离子自由基含量先降后增；菖蒲SOD活性与苯丙氨酸解氨酶（PAL）活性均随着铬（Ⅵ）浓度的增加呈先增后降趋势，而CAT活性和POD活性均显著增加；综上，菖蒲能适应一定浓度的铬胁迫并做出相应的调节，从而表现出较强的适应力和耐污力，可以作为人工湿地处理含铬废水的物种之一。
Taking Acorus calamus plants with the same of activity and size as materisl,effect of Cr （VD stress （0,5,10, 20,40,60 mg/L） on the physiological and biochemical index （such as TTC, chlorophyll content, vitamin C content, Proline content, MDA content, SOD activity, POD activity, CAT activity and PAL activity） of Acorus calamus were studied in order to provide theoretic foundation for phytoremediation of Cr contaminated waters. The results showed that chlorophyll contents increased in the leaves of Acorus calamus under lower Cr （Ⅵ） stress （5, 10, 20 mg/L）, while decreased significantly under higher Cr （Ⅵ） stress （40,60 mg/L）. With Cr （Ⅵ） stress deepen, soluble protein content increased significantly, while soluble sugar content was decreasing as a whole, and the contents of Proline, TTC, GSH and MDA firstly increasing and then decreased with the increasing of Cr （Ⅵ） stress. And superoxide free radical contents firstly decreased and then increased, while Vitamin C content decreased under Cr （Ⅵ） stress. For antioxidant enzyme activity, SOD activity and PAI. activity firstly increased and then decreased with the increasing of Cr concentrations,while CAT activity and POD activity increased significantly. Therefore,it could be concluded that Acorus calamus could adapt the certain concentration of Cr stress and make corresponding adjustments. Then Acorus calamus had a good adaptability and pollution resistance ability to Cr （VI） stress, which was an excellent constructed wetland ptant in the treatments of Cr wastewaters.
出处
《北方园艺》
CAS
北大核心
2014年第3期54-59,共6页
Northern Horticulture
基金
教育部新世纪人才支持计划资助项目(NCET-12-0659)
贵州省优秀科技教育人才省长资金资助项目(黔省专合字[2012]71号)
贵阳市低碳科技计划资助项目(筑科合同[2012205]号)
贵州省教育厅科技创新人才支持计划资助项目(黔财教[2012]224号)
贵州省科学技术基金资助项目(黔科合J字LKM[2012]05号
黔科合J字[2011]2363)
关键词
铬胁迫
菖蒲
生理生化
人工湿地
环境修复
Cr（Ⅵ） stress
Acorus calamus
physiology and biochemistry
constructed wetlands
environmental remediation
