摘要
目的探讨外周血Th17/Treg失衡与儿童过敏性紫癜(HSP)合并肺炎支原体(MP)感染的关系。方法选择18例MP-IgM阳性HSP患儿(HSP-MP组)、40例MP-IgM阴性HSP患儿(HSP组)、23例MP-IgM阳性非HSP患儿(MP组)、19例健康体检儿童(对照组),比较各组采用流式细胞术(FCM)检测的外周血Th17/Treg细胞百分率,酶联免疫吸附(ELISA)法检测的血清IL-17、1L-6、TGF-β水平。结果 HSP-MP组的外周血Th17细胞、IL-17、IL-6水平高于HSP组、MP组及对照组,HSP组及MP组亦高于对照组,差异均有统计学意义(P均<0.05);HSP-MP组的Treg细胞水平低于HSP组、MP组及对照组,HSP组及MP组亦低于对照组,差异均有统计学意义(P均<0.05);HSP组与MP组间差异无统计学意义(P>0.05)。各组间TGF-β水平的差异无统计学意义(P>0.05)。结论 Th17/Treg免疫失衡可能参与了MP-IgM阳性HSP的发病过程。
Objectives To investigate the role of Thl7/Treg cells imbalance in the pathogenesis of Henoch-Schonlein purpura （HSP） complicated by Mycoplasma pneumoniae （MP） infection in children. Methods Eighteen MP-IgM-positive HSP children （HSP-MP group）, 40 MP-IgM-negative HSP children （HSP group）, 23 MP-IgM-positive non- HSP children （MP group） and 19 healthy children （control group） were enrolled. The percentages of Thl 7 and Treg cells in peripheral blood in all objects were detected by flow cytometric analysis （FCM）, and serum levels of IL-17, IL-6 and TGF-[3 were measured by ELISA. Results The percentage of Thl7 cells and the levels of IL-17 and IL-6 in HSP-MP group were significantly higher than those in other three groups （P〈0.05）. The percentage of Th17 cells and the levels of IL-17 and IL-6 in HSP group and MP group were significantly higher than those in control group （P〈0.05）. The percentage of Treg cells in HSP-MP group were significantly lower than those in other three groups （P〈0.05）. The percentage of Treg cells in HSP group and MP group were significantly lower than those in control group （P〈0.05）. There was no significant difference in the percentage of Thl7 and Treg cells and the levels of IL-17 and IL-6 between HSP group and MP group （P〉0.05）. There was no significant difference in level of TGF-[3 among the four groups （P〉0.05）. Conclusions The imbalance of Th17/Treg cells in peripheral blood may contribute to the development of HSP complicated by MP infection in children.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2014年第1期8-11,共4页
Journal of Clinical Pediatrics
基金
滨州市科技发展计划项目(No.2011ZC0909)
滨州医学院科技计划项目(No.BY2011KJ005)
