摘要
目的探讨调节性T细胞（Treg细胞）与Th17细胞失平衡在特应性皮炎（AD）发病机制中的作用。方法采用流式细胞仪检测41例AD患者外周血Treg细胞与Th17细胞的比例，实时定量逆转录聚合酶链反应检测Treg细胞与Th17细胞转录因子Foxp3、RORγtmRNA的表达水平，酶联免疫吸附试验检测血清中转化生长因子β（TGF-β）、白介素17（IL-17）和白介素23（IL-23）的含量；同时以38例性别、年龄匹配的健康儿童作为对照组。采用独立样本t检验和线性相关进行统计学分析。结果AD患者组外周血Treg细胞（CD4＋CD25＋Foxp3＋T细胞）比例为2．01％±0．57％，Foxp3mRNA的表达量为0．65±0．19；健康对照组分别为5．04％±1．44％和1．71±0．69，AD患者组均明显低于健康对照组（两组比较，t值分别为12．47和9．47，P值均〈0．01）。AD患者组外周血Th17细胞（CD4＋IL-17＋T细胞）比例为1．77％±0．55％，RORγtmRNA表达量为5．97±1．45；健康对照组分别为0．39％±0．15％和1．49±0．57，AD患者组均显著高于健康对照组（两组比较，t值分别为14．82和17．78，P值均〈0．01。AD患者组Treg／Th17细胞比值（1．26±0．61）较健康对照组（14．53±5．77）明显降低（t=14．11，P〈0．01）。急性期AD患者Treg／Th17细胞比值为0．88±0．04，与亚急性期（1．29±0．11，t=4．02，P〈0．01）和慢性期（2．05±0．24，t=4．83，P〈0．01）AD患者比较，差异均有统计学意义；AD患者亚急性期号幔性期比较，差异亦有统计学意义（t=2．89，P〈0．05）。AD患者血清中IL-17和IL-23含量均高于健康对照组（P值均〈0．01），而TGF—B含量比较，差异无统计学意义（t=1．96，P〉0．05）。中、重度AD患者疾病严重程度评分与外周血Treg细胞比例呈负相关（r=-0．40，P〈0．05），与Th17细胞比例呈正相关（r=0．42，P〈0．05）。结论AD患者外周血Treg和Th17细�
Objective To assess the role of imbalance between regulatory T （Treg） cells and T helper 17 （Th17） cells in the pathogenesis of atopic dermatitis （AD）. Methods Peripheral blood was obtained from 41 patients with AD and 38 age-and sex-matched healthy controls. Flow cytometry was performed to determine the percentage of Treg cells （CD4＋CD25＋Foxp3＋ T cells） and Thl7 cells （CD4＋IL17＋T cells）, real-time quantitative reverse transcription （RT）-PCR to detect the mRNA expressions of Foxp3 and RORγt, which are the specific transcription factors of Treg and Th17 cells respectively. Serum concentrations of transforming growth factor （TGF）-β, IL-17 and IL-23 were measured by enzyme linked immunosorbent assay （ELISA）. Data were statistically assessed by independent-samples t test and Pearson correlation analysis. Results The patients with AD showed an obvious decrease in Treg cell percentage, transcription factor Foxp3 mRNA level and Treg/ Thl7 ratio （2.01%±0.57% vs. 5.04%±1.44%, t = 12.47, P〈 0.01; 0.65±0.19 vs. 1.71±0.69, t = 9.47, P〈 0.01; 1.26±0.61 vs. 14.53±5.77, t = 14.11, P 〈 0.01）, but a significant increase in peripheral Thl7 cell percentage and transcription factor RORγt mRNA level （1.77%±0.55% vs. 0.39%± 0.15%, t = 14.82, P 〈 0.01; 5.97±1.45 vs. 1.49±0.57, t = 17.78, P 〈 0.01） compared with the healthy controls. Further comparison revealed that Treg/Th17 ratio was significantly lower in patients with acute AD than in those with subacute AD （0.88±0.04 vs. 1.29±0.11, t = 4.02, P〈 0.01） and those with chronic AD （2.05±0.24, t = 4.83,P〈 0.01）,statistically different between patients with subacute AD and chronic AD （t = 2.89,P 〈 0.05）. There was no significant difference in the serum concentration of TGF-β between patients with AD and healthy controls （（15.28±2.34） μg/L vs. （16.56±3.27） μg/L, t = 1.96, P〉 0.05）. A significant increase was observed in the serum levels of IL-17 and IL-23 in patients with AD com
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2012年第7期481-484,共4页
Chinese Journal of Dermatology
基金
山东省科技发展计划（2011YD18069）
山东省自然科学基金（ZR2010HQ013）
山东省高等学校科技计划项目（J10LF90）
山东省医药卫生科技发展计划（2011QZ003）
滨州市科技发展计划（2011ZC0914）
