摘要
目的:探讨新疆维吾尔族和汉族乳腺癌组织中哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及其下游分子4EBP1和S6K1的表达差异。方法:选取2005-03-01-2009-09-30新疆医科大学附属肿瘤医院的维吾尔族和汉族女性浸润性乳腺癌患者285例,使用免疫组化方法检测磷酸化mTOR及其下游分子4EBP1和S6K1的表达状况;分别选取2012-01-01-2012-06-30新疆医科大学附属肿瘤医院63例维、汉新鲜乳腺癌和癌旁组织样本,使用荧光定量RT-PCR法检测mTOR及其下游分子4EBP1和S6K1的mRNA表达状况。结果:免疫组化结果显示,维吾尔族和汉族p-mTOR在乳腺癌组织中的阳性表达率分别为77.1%和67.4%(P=0.067),在癌旁组织中的阳性表达率分别为57.6%和61.7%(P=0.485);p-4EBP1在癌组织中的阳性表达率分别为31.9%和20.0%(P=0.020),在乳腺癌旁组织中的阳性表达率分别是4.9%和12.1%(P=0.029);p-S6K1在癌组织中的阳性表达率分别为41.0%和43.3%(P=0.695),在乳腺癌旁组织中的阳性表达率分别为14.0%和22.7%,P=0.054;荧光定量RT-PCR结果显示,维、汉乳腺癌组织中mTOR mRNA相对定量结果差异有统计学意义,P=0.045;4EBP1和S6K1mRNA相对定量结果差异无统计学意义,P值分别为0.128和0.404。结论:mTOR信号通路中p-4EBP1的表达水平存在维、汉民族的差异,为研究维、汉不同民族之间乳腺癌差异及个体化治疗提供了进一步研究方向。mTOR及其下游分子mRNA表达水平与磷酸化蛋白的表达并不完全一致,提示蛋白表达的调控位点可能位于转录后水平。
OBJECTIVE：To discuss the expression of roTOR and its downstream molecular 4EBP1 and S6K1 in of Xinjiang Uygur and Han breast cancer patients. METHODS： Totally 285 cases of breast cancer patients,who were treated in Affiliated Tumor Hospital of Xinjiang Medical University from March 1,2005 to September 30,2009, were randomly selected to detect the expression of p-mTOR, p 4EBP1 and p-S6K1 using immunohistochemistry. Sixty-three cases of breast cancer and paracancerous tissue were reserved respectively to detect the mRNA expression using RT-PCR technology. RESULTS： Results from immunohistochemistry of uygur and Han showed that the positive p-mTOR were 77.1% and 67.4% in breast cancer（P=0. 067）,57.6% and 61.7% in normal tissue （P=0. 485） ;p-4EBP1 were 31.9% and 20.0% （P=0. 020） in breast cancer,4.9% and 12. 1%（P=0. 029） in normal tissue;p-S6K1 were 41.0% and 43.3% in breast cancer （P=0. 695） ,14.0% and 22.7% in normal tissue（P=0. 054）. There was statistical difference of p-mTOR mRNA expression between Uygur and Han （P=0. 045）, whereas p-4EBP1 and p-S6K1 mRNA were not significantly different （P value were 0. 128 and 0. 404 respectively）. CONCLUSIONS： The higher expression of p-4EBP1 in Uygur breast cancer tissue may provide a basis for the further research of finding the disparities between ethnic groups and individualized treat ment. The regulation of activated protein expression in mTOR signaling pathway may be post transcriptional,in respect of the disaccord of mRNA with activated protein expression.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第23期1788-1793,共6页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30960376)
