摘要
首次采用主动条件性回避反应模型探讨了 Fyn-激酶缺损的突变型小鼠的学习能力。以光 (40 W)为条件刺激 ,以电击为非条件刺激。以能驱使小鼠跑向安全区的最低电压作为电击的强度。以 1 0次条件刺激中有 9次出现条件性回避反应定为学会标准。实验结果表明 ,纯合子 Fyn-激酶缺损小鼠需经 (79.1 3±30 .1 0 )次训练才能达到学会的标准 ,而杂合子 Fyn-激酶缺损小鼠和野生型小鼠 (C57BL/6)分别只需经(2 7.38± 7.86)次和 (34.87± 1 0 .90 )次训练即能达到学会标准。纯合子组分别与其他两组小鼠间的差别均具有统计的显著性 (P<0 .0 0 5)。然而 ,纯合子 Fyn-激酶缺损小鼠的两周记忆保持与其他两组相比没有显著性差别。根据以上实验结果可以认为 ,Fyn-激酶缺损可导致小鼠的联合型学习能力下降。同时提示 。
In the present study the conditioned avoidance training was carried out in an one way avoidance box.Light(40W) was used as the conditioned stimulus,and the electric shock as the unconditioned stimulus.In order to avoid eliciting hyperreactivity to aversive stimulus,the minimal intensity of electric shock for driving the mouse to run to the safe compartment was used.The results showed that homozygous Fyn kinase deficient mice needed much more trials (79 13±30 10)for reaching the learning criterion than other two groups did.The trials needed to reach the learning criterion for heterozygous and wild type groups were 27 38±7 86 and 34 87±10 90,respectively.The differences between homozygous and other two groups were very significant( P <0 005).However,no deficit of 2 week memory retention of homozygous Fyn kinase deficient mice was found.On the basis of these results it may be concluded that Fyn deficiency impairs associative learning.Moreover,it is supposed that the behavioral model of conditioned avoidance task is useful for behavioral study of mutant mice. [
基金
国家自然科学基金!(396 30 14 0 )资助项目
