摘要
研究应用定性PCR技术对山东菜区22个不同番茄品种和一个阳性对照、一个阴性对照中外源基因35S启动子、NPTⅡ基因和NOS终止子进行检测。实验结果表明,阳性对照可以稳定扩增到预期大小的片段,而21个待检测品种和阴性对照则没有扩增到195 bp、180 bp特异片段。待检品种瑞芬扩增到195 bp、180 bp、263 bp和274 bp的特异片段,初步确定该品种含有可疑外源基因。
Using qualitative PCR technique, the exogenous gene 35S promoter, NPT II gene and NOS terminator detection of 22 tomato cultivars in Shandong Province was conducted, with one tomato cultivar （HuMan No.l） using as positive control and one tomato cuhivar （Maofen No.802） using as negative control. Results showed that the expected DNA segment can be obtained steadily through amplification. However, 21 tomato cuhivars and the negative control can not be got through amplification. The specific DNA segments of 195 bp, 180 bp, 263 bp and 274 bp was amplified from Ruifen tomato cuhivar, which preliminarily indicated that the exogenous gene was in genome of the tomato cultivar.
出处
《食品研究与开发》
CAS
北大核心
2013年第22期52-54,共3页
Food Research and Development
