摘要
目的研究姜黄素对原代培养的肺巨噬细胞表达TLR4mRNA及分泌TNF-a、IL-6和IL-8的影响。方法用脂多糖(LPS)建立体外肺巨噬细胞体外炎症损伤模型,用ELISA观察肺巨噬细胞分泌TNF-a、IL-6和IL-8和用RT-PCR、western blot观察肺巨噬细胞TLR4的表达。结果 LPS明显诱导肺巨噬细胞分泌TNF-a、IL-6和IL-8增加及TLR4的增量表达(P<0.01),姜黄素可抑制这种作用(P<0.05),且与姜黄素药物浓度有关(P>0.05)。结论姜黄素对肺巨噬细胞的抗炎机制可能是通过抑制TLR4的表达,通过脂多糖-TLR4信号传导通路,从而减少炎性细胞因子的释放;姜黄素有可能作为一种有研究潜力的抗感染治疗的中药。
Objective To study the influence of Curcumin on the expressions of TLR4 and TNF-a、IL-6 、IL-8 release in pulmonary macrophage. Methods The experiments were performed in culture of LPS induced pulmonary macrophage in vitro,the TNF-a、IL-6 、IL-8 release was detected with ELISA and the expressions of TLR4 was detected with RT-PCR and western blot. Results Pulmonary macrophage was stimulated with LPS and the expressions of TLR4 and TNF-a 、IL-6、IL-8 release was increased （P〈0.01）,but it was inhibited by Curcumin （P〈0.05）,and dose dependence （P〈0.05）. Conclusion The mechanism of Curcumin’s antiinflammatory may be reduce cytokine release,by inhibits expressions of TLR4 and via Cross talk toll-like receptor-4 signaling;Curcumin may be used as a kind of anti-infective drug.
出处
《云南中医学院学报》
2013年第6期22-25,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
重庆市自然科学基金计划项目(NO:cstc2013jcyjA1062)
