摘要
目的研究理冲汤对异种移植子宫肌瘤小鼠免疫相关因子单核细胞趋化因子-1(MCP-1)和细胞间黏附分子-1(ICAM-1)表达的影响,为临床治疗提供科学依据。方法将50只6~9周龄CB-17Scid小鼠随机(随机数字表)分成5组,即正常组、模型组、理冲汤高剂量组(30 g/kg)、理冲汤低剂量组(10 g/kg)、化药对照组(米非司酮2.9 mg/kg),通过异种移植的方法制备小鼠子宫平滑肌瘤动物模型,药物干预4周后,HE染色光镜观察小鼠子宫平滑肌的病理变化,采取免疫组化检测小鼠子宫平滑肌MCP-1、ICAM-1的表达水平。结果 HE染色光镜显示,模型组与正常组相比,呈活跃增生现象,与人体肌瘤样增生一致,提示造模成功。免疫组化结果显示,理冲汤高低剂量组可显著降低小鼠子宫肌瘤组织中MCP-1、ICAM-1的表达,与模型组相比差异有统计学意义(P<0.05)。结论理冲汤能显著降低子宫肌瘤平滑肌中MCP-1、ICAM-1的表达,进而对子宫肌瘤起到抑制作用。
Objective To study the impacts of lichong decoction on the expressions of MCP-1 and ICAM-1 in the mice of heterograft uterine fibroids so as to provide the scientific evidence for clinical treatment. Methods Fifty CD-17 Scid mice aged from 6 to 9 weeks were randomized into five groups,i. e. a normal group,a model group,a high-dose lichong decoction group（ 30 g/kg）,a low-dose lichong decoction group（ 10 g/kg） and a chemical drug control group（ mifepristone 2. 9 mg/kg）. With heterograft method,the mouse model of uterine fibroids was prepared. After drug intervention for 4 weeks,HE staining was used to observe the pathological changes of uterine fibroids in mice. IHC method was used to determine the expressions of MCP-1 and ICAM-1 in uterine smooth muscle. Results HE staining results showed that compared with the normal group,the hyperplasia was active,same as human myomatous hyperplasia,indicating the success of modeling. IHC results showed that the high-dose and low-dose lichong decoction reduced significantly the expressions of MCP-1 and ICAM-1 in uterine fibroids tissue,presenting the significant differences as compared with the model group（ P〈0. 05）. Conclusion Lichong decoction significantly reduces the expressions of MCP-1 and ICAM-1 in smooth muscle of uterine fibroids,and further inhibits on uterine fibroids.
出处
《世界中西医结合杂志》
2017年第1期53-56,共4页
World Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金项目(81373812)
国家自然科学基金项目(81073096)
