摘要
利用PCR和RT—PCR方法对豫南地区213个不同规模猪场收集的病料和血清分别进行病原体检测,以调查猪瘟(CSF)、猪圆环病毒病(PCVD)、猪繁殖与呼吸综合征(PRRS)和猪伪狂犬病(PR)的流行情况。结果显示:猪场中感染阳性率最高的是PCV-2,为70.5%,最低的均是PR,为32.4%;通过对猪场中4种病毒之间的混合感染调查中发现,该地区混合感染情况比较严重,74.6%的猪场存在2种或2种以上病毒混合感染。并且证实该地区PRRSV和HPRRSV均是流行毒株,并且在同一猪场同时检出这两个毒株的几率很大。以上结果呈现了4种疫病在该地区的感染情况和流行规律,以期为该地区养猪业提供参考,从而采取合理有效的防治措施。
In order to investigate the prevalence status of classical swine fever （CSF）, porcine circovirus disease （PCVD）, por- cine reproductive and respiratory syndrome （PRRS） and porcine pseudorabies （PR）, specimen and serum samples collected from 213 farms of different sizes in southern regions of Henan province were detected for pathogens by PCR and RT-PCR methods The results indicated that PCVD showed the highest positive incidence among four virus diseases which was 70.5%, and PR showed the lowest positive rate which was 32.4%. It was found that two or more viruses were detected in the same specimen and serum sample from 74.6% farm through investigating mixed infection from four viruses. In addition, the results also indicated that present PRRSV and HPRRSV were popular strains and extremely high incidence of both strains detected on the same farm. All the results above clearly showed the infectious status and epidemics of the four diseases, providing the reference for prevention and controt of these diseases.
出处
《中国兽医杂志》
CAS
北大核心
2013年第12期14-17,共4页
Chinese Journal of Veterinary Medicine
基金
信阳农业高等专科学校青年教师科研基金项目(2009ZRKX016)
