摘要
从新疆辐射污染区采集的40份土样中,通过重金属离子压力富集筛选,获得46株细菌。通过序列比对、聚类分析,确定46株菌中16株为芽孢杆菌属(Bacillus),13株类芽孢杆菌属(Paneibacillus),1株欧文菌属(Erwinia),10株假单孢菌属(Pseudomonas),1株不动杆菌属(Acinetobacter),1株克氏菌属(Kocuria),1株泛菌属(Pantoea),1株沙雷氏菌属(Serrolia),2株链霉菌属(Streptomyces)。通过进一步的重金属耐性分析,结果显示菌株对5种重金属离子的耐受程度总体呈现Pb2+>Zn2+>Co2+>Hg2+>Cu2+的趋势,其中对Pb2+的最大耐受性可达2 100 mg/L,对Zn2+的最大耐受性可达1 200 mg/L,对Co2+的最大耐受性可达1 000 mg/L,对Hg2+的最大耐受性可达500 mg/L,对Cu2+的最大耐受性可达450 mg/L。表明干旱荒漠土壤中具有较丰富的耐重金属细菌属种多样性,为耐辐射微生物在重金属污染治理方面的应用提供理论依据。
46 bacteria were enriched screened and isolated by the pressure of heavy metal ions from 40 radiation-polluted soil samples. Using 16S rRNA gene sequence and clustering analysis, results showed that among 46 bacteria, 16 strains were Bacillus, 13 strains were Paneibacillus, 1 strain was Erwinia, 10 strains were Pseudomonas, 1 strain was Acinetobacter, 1 strain was Kocuria, 1 strain was Pantoea, 1 strain was Serrolia, 2 strains were Streptomyces. By further analysis on heavy metal tolerance, results showed that the tolerance trend of 5 heavy metals ions was Pb2~〉Zn2＇〉Co2＇〉Hg2~〉Cu2~, with the highest tolerability to Pb2~ and Zn2＋ respectively up to 2 100 mg/L and 1 200 mg/L, followed tolerability to Co2＋ as 1 000 mg/L, tolerability to Hg2＋, Cu2＋ respectively up to 500 mg/L and 450 mg/L, which indicated a rich diversity of heavy metal resistant bacteria in the radiation-polluted soil. The assay would provide a theoretical basis and technological foundation for further study of the radiation-resistant microorganisms in the heavy metal pollution.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2013年第12期29-32,共4页
Environmental Science & Technology
基金
国家"863"计划项目(2012AA021705
2012AA063503)
关键词
辐射污染区土壤
耐重金属细菌
多样性
研究
radiation-polluted soil
heavy metal resistant bacteria
diversity
investigation
