摘要
为了解Cr（Ⅵ）对芽孢杆菌（Bacillus sp.FG-01）降解苯酚的影响,探讨了Cr（Ⅵ）存在下,菌液量、p H值、Cr（Ⅵ）质量浓度、苯酚质量浓度对菌株FG-01降解苯酚的影响,分析了其降解动力学;以壳聚糖为原料,采用包埋法制备了固定化FG-01菌小球,并将其与游离菌在Cr（Ⅵ）存在下降解苯酚进行了比较。结果表明：Cr（Ⅵ）存在时对菌株FG-01降解苯酚有一定的抑制作用;Cr（Ⅵ）质量浓度小于5 mg/L时,随着苯酚质量浓度增大,菌株FG-01对苯酚的降解速率变慢;降解的适宜p H值为5.18~8.0;在Cr（Ⅵ）质量浓度1~4 mg/L时,菌株FG-01降解苯酚的过程符合零级动力学方程;降解温度、苯酚质量浓度等相同时,抑制作用Cr（Ⅵ）〉Zn（Ⅱ）;固定化FG-01菌小球在抑制作用较强的Cr（Ⅵ）存在下对苯酚的降解速率与对Cr（Ⅵ）的耐受程度均高于游离菌。
The effects of the amount of bacteria, p H value, concentration, carbon source on degradation of phenol by Bacillus sp. FG-01 with coexistence of Cr（Ⅵ） were investigated. The kinetic of phenol degradation by Bacillus sp. FG-01 was discussed. The degradation performances by the biomaterial based on the beads with immobilized Bacillus sp. FG-01 in chitosan, were compared with those of free cells. It was concluded that chromium（Cr（Ⅵ）） had a restraining effect on degradation of phenol by Bacillus sp. FG-01. The rate of phenol biodegradation with Cr（Ⅵ） coexistence was decreased with the increasing of phenol concentration when the concentration of Cr（Ⅵ） was less 5 mg/L. The suitable p H for degradation of phenol by Bacillus sp. FG-01 was between 5.18~8.0. The kinetic of phenol biodegradation could well accord with the zero order equation for phenol as the sole carbon source with 1~4 mg/L Cr（Ⅵ） coexistence. The inhibition degree is in the order of Cr（Ⅵ）〉 Zn（Ⅱ） in the same temperature and concentration of phenol. Compared with free cells, the method of using Bacillus sp. FG-01 immobilized in chitosan has a higher phenol removal ability and capacity for tolerance to Cr（Ⅵ）.
出处
《非金属矿》
CAS
CSCD
北大核心
2015年第6期69-72,78,共5页
Non-Metallic Mines
基金
国家自然科学基金青年基金(201301126)
山西省青年科技研究基金(2013021009-3)
山西省科技发展计划工业攻关项目(20130321033-02)
太原市科技项目(120238)
山西省科技攻关项目(02010145)
关键词
芽孢杆菌
苯酚
生物降解
Cr(Ⅵ)
固定化
Bacillus sp.
phenol
biological degradation
Cr（Ⅵ）
immobilization
