摘要
运用生物信息学方法分析了猪和其他21个物种CB1基因CDs序列的系统进化关系和猪CB1基因编码蛋白质的理化性质与结构。结果显示,CB1基因同源性较高,且在进化中受到纯化选择的作用。猪CB1蛋白为疏水性跨膜蛋白,包含472个氨基酸残基,不含信号肽。其一级结构含有23个磷酸化位点、6个糖基化位点;二级结构含有47.67%的α螺旋、39.62%的无规则卷曲、12.71%的延长链;三级结构由7个α螺旋和无规则卷曲组成。研究结果表明,CB1基因可能是哺乳动物的看家基因,7条相连的α螺旋结构是猪CB1的活性位点。
In the study,the phylogenetic relationship of the coding sequences （CDS） of CB1 gene between swine and other 21 species,and the physicochemical characters and structural properties of CBl-encoding protein in swine were analyzed with bioinformatics methods. The results showed that the homology of CB1 gene was high as purifying selection could exist in its evolution. The CB1 protein was a hydrophobic transmembrane protein consisting of a,72 amino acid residues without signal peptide. The primary structure of the protein CB1 contained 23 phosphorylation sites and 6 glycosylation sites, the secondary structure was made up of 47. 67% of a-helix, 39.62 % of random coil, 12.71 % of extended strand, the tertiary structure was composed of 7 α-heli- ces and random coil. The results indicate that CB1 maybe is a housekeeping gene of mammals and the 7 connected α-helices are active sites of CB1 in swine.
出处
《河南农业科学》
CSCD
北大核心
2013年第12期129-132,共4页
Journal of Henan Agricultural Sciences
基金
河北省现代农业产业技术体系创新团队建设专项资金项目
