摘要
目的 :寻找肝细胞癌微小转移的标志物。方法 :用巢式反转录聚合酶链反应 (Nested- RT- PCR)技术检测 6 5例肝细胞癌、2 1例非肝癌的恶性肿瘤、2 2例慢性乙型肝炎或肝硬化患者及 2 1例健康志愿者外周静脉血中的甲胎蛋白 (AFP) m RNA。结果 :AFP- m RNA在健康志愿者、非肝癌恶性肿瘤患者外周血中均为阴性 ;在肝细胞癌组外周血中 AFP- m RNA的检出率 (4 4/6 5 ,6 7.7% )明显高于慢性乙型肝炎或肝硬化组 (2 /2 2 ,9.1% ,P<0 .0 1)。AFP- m RNA检出率与临床分期、门静脉癌栓、肝外转移显著相关。在 12例血清 AFP<2 5 μg/L 的肝癌患者血中 ,7例 (5 8.3% )可检出 AFP- m RNA。结论 :AFP- m RNA可作为血循环中有肝癌细胞或肝细胞的标志物 ,在肝癌患者 ,阳性预示有血源性转移的可能 ;并且 AFP- m RNA对血清
Objective:To search the marker of micrometastatic hepatocellular carcinoma (HCC).Methods:Peripheral blood samples were obtained from 65 patients with hepatocellular carcinoma,21 non HCC malignant tumors,22 chronic hepatitis B or cirrhosis,and 21 normal healthy volunteers.To identify hepatocellular carcinoma cells in peripheral blood, liver specific alpha fetoprotein(AFP)mRNA was amplified from total RNA extracted from whole blood by nested reverse transcription polymerase chain reaction (Nested RT PCR).Results:AFP mRNA was not detected in the normal healthy volunteers and patients with non HCC malignant tumors.The presence of AFP mRNA in patients with HCC(44/65,67.7%)was higher than those with chronic hepatitis B or cirrhosis(2/22,9.1%, P <0.01).The detective rate of AFP mRNA in blood seemed to be correlated with the clinical stages of HCC,the presence of extrahepatic metastasis,and portal vein thrombosis.AFP mRNA was detected in 7 of 12(58.3%)HCC patients with low level of serum AFP(<25 μg/L).Conclusion:The presence of AFP mRNA in peripheral blood may be an indicator of malignant or benign hepatocytes,which might predict hematogenous spreading metastasis of tumor cells in patients with HCC,and it might play a supplementary role in the diagnosis of HCC with negative or low level of serum AFP.
出处
《肿瘤研究与临床》
CAS
2000年第6期363-366,共4页
Cancer Research and Clinic
基金
广西自然科学基金资助项目!(9732 0 2 9)
