摘要
目的探讨乳腺癌患者外周血中小黏蛋白(SBEM-mRNA)的表达与乳腺癌微转移的关系。方法利用Nested-RT-PCR技术检测92例乳腺癌术后患者,20例乳腺良性肿瘤患者,10例其他系统恶性肿瘤患者,10例健康人群外周血SBEM-mRNA的表达。结果乳腺癌组外周血SBEM-mRNA表达率为46.7%(43/92),其他各组均为阴性。SBEM表达与ER和PR状态、患者月经状况和肿瘤大小无关;与CEA,CA15-3和Her-2在外周血中的表达状况、患者年龄、腋窝淋巴结转移状况有关,上述5因素分组间差异有统计学意义(P<0.05和0.01)。结论外周血中SBEM-mRNA的表达可作为乳腺癌微转移的一个辅助检测指标。
Objective To study the expression of small breast epithelial mucin （ SBEM ） in the peripheral blood of the patients with breast cancer, and explore the correlation between micrometastasis and SBEM. Methods Peripheral blood samples were obtained from 92 patients with breast carcinoma, 20 patients with benign breast diseases, 10 patients with malignant tumor not originating from breast, and 10 normal healthy volunteers. SBEM-mRNA expression was detected by nested reverse transcription-polymerase chain reaction （Nested-RT PCR）. Results Among 92 breast carcinoma patients, 43 （46. 7% ） were Nested-RT-PCR positive for SBEM-mRNA, but it was negative in all the other groups. The expression of SBEM-mRNA in peripheral blood did not correlate with ER or PR status, menstruation state or primary tumour size. but correlated with patient＇s age, the state of Her-2 ,CEA, CA15-3 and axillary lymph node metastasis of breast cancer. Conclusions The expression of SBEM-mRNA in peripheral blood of breast cancer can be of potential use to monitor the micrometastasis of breast cancer.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第11期1122-1125,共4页
China Journal of General Surgery
