摘要
建立了简便、快速测定花生中白藜芦醇的在线固相萃取富集-高效液相色谱法。固相萃取富集柱填料为dsDNA，在线固相萃取富集过程的上样溶剂为纯水，淋洗溶剂为纯水，转移溶剂为乙腈-水（80：20，V/V），分析测定溶剂为乙腈-水（25：75，V／V）。本方法对白藜芦醇检测的线性方程为Y=1843．805X＋39．1791，相关系数为0．99978；检出限（LOD）为0．004mg／kg；定量限（LOQ）为0．01mg／kg；基质4个水平添加回收率（n=6）在92．48％-107．98％；6次加标回收相对标准偏差（RSD）在0．19％～6．05％。
A novel method was developed to determine resveratrol in peanut by HPLC with on -line solid phase extraction. The on - line soild phase extraction column was prepared by packing dsDNA column filler in stainless column. The loading and washing solution of the extraction column were both water, the transferring solu- tion was acetonitrile -water （80： 20, V/V）, and the determining solution was acetonitrile -water （25： 75, V/V）. Under the above condition, the linear regress equations was Y = 1 843. 805X ＋ 39. 179 1, with correlation coeffi- cients of 0.999 78, detection limits of 0.004mg/kg, and quantification limits of 0.01mg/＇kg. The average recoveries of 4 levels spiking were 92.48% to 107.98%, the relative standard deviation （RSD） was 0.19% to 6.05% （ n =6）.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第6期712-716,共5页
Chinese Journal of Oil Crop Sciences
基金
公益性行业(农业)科研专项(201303088)
国家科技支撑计划(2012BAK08B03)
"双打"研究专项(2012104010-4)
湖北省自然科学基金(2011CDB356)
关键词
在线固相萃取富集
高效液相色谱法
花生
白藜芦醇
On - line solid phase extraction
High performance liquid chromatography （ HPLC ）
Peanut
Res- veratrol
