摘要
土壤微生物群落被认为是土壤生态系统变化的预警及敏感指标,指示着土壤质量的变化。本研究利用3年定位试验,通过测定磷脂脂肪酸(PLFAs)的含量,分析了连年绿肥和化肥配施对植烟土壤微生物群落结构的影响。研究结果表明:施肥明显地提高了土壤中PLFAs的种类和总量;在绿肥和化肥配施处理中,当翻压15 000 kg/hm2绿肥的基础上施用大于85%常规化肥时,明显提高了土壤中细菌、AM真菌及微生物总的PLFAs含量;而当化肥的施用量减至常规施肥70%时,对土壤细菌、真菌及微生物总PLFAs含量的积累产生不利影响。从不同处理的主成分分析和聚类分析的结果可知,不同绿肥和化肥配施比例对土壤微生物群落结构变化有明显影响。经相关性分析表明,PLFA分析方法和氯仿熏蒸法之间具有很好的一致性,且土壤速效钾和速效磷含量与革兰氏阴性菌的PLFAs含量呈显著相关,而与土壤真菌PLFAs含量则无明显的相关性。
Soil microbial community can indicate soil quality change and is regarded as the early warning and the sensitive indicator. In order to study the effects of green manure application combined with chemical fertilizers on soil microbial community structure of tobacco-planting soils, the phospholipid fatty acids （PLFAs） were determined in 3a located experiment. The results showed that fertilization increased the kinds and amounts of soil PLFAs. The bacterial PLFAs, arbuscular mycorrhizal fungi PLFAs and total soil microbial PLFAs were enhanced distinctly when the amount of chemical fertilizers used more than 85% of conventional dosage with burying 15 000 kg/hm^2 green manure. The negative effect on soil microbial community structure was appeared when the amount of chemical fertilizers was used less than 70% of conventional dosage with burying 15 000 kg/hm^2 green manure. Principal component analysis and cluster analysis indicated that soil microbial community structure changed due to the ratio of green manure and chemical fertilizers. Correlation analysis showed the high agreement between chloroform-fumigation extraction and PLFA methods, available potassium and phosphorus were significantly positively correlated with the G-PLFAs, but not correlated with fungi PLFAs.
出处
《土壤》
CAS
CSCD
北大核心
2013年第6期1070-1075,共6页
Soils
基金
国家公益性行业(农业)科研专项(200803029-01-08)
国家烟草专卖局项目(110201202014)
湖北省农业科技创新中心项目(2007-620-001-03)资助
关键词
绿肥
植烟土壤
磷脂脂肪酸
微生物群落结构
Green manure, Tobacco-planting soils, Phospholipid fatty acids, Microbial community structure
