摘要
为弄清海南薯蓣茎腐病病原,采用离体致病性测定、形态学和分子生物学鉴定等方法,对其进行了研究。从海南临高、海口等薯蓣生产基地采集病样89份,纯化出分离物28个。经致病性试验证实,分离物lp3-1、bl6-5、bd6-4和lp2-5为薯蓣茎腐病病原菌。经形态学鉴定,4个分离物均为镰刀菌(Fusarium sp.)。利用真菌18S rRNA基因引物、镰刀菌特异性引物和轮枝镰刀菌特异引物进行PCR扩增,能扩增出510 bp大小的镰刀菌DNA片段,不能扩增出轮枝镰刀菌DNA片段。序列分析表明:分子生物学和形态学鉴定结果一致,且镰刀菌Fu3/Fu4区序列分析能进一步确定薯蓣茎腐病是由尖孢镰刀菌薯蓣专化型(Fusarium oxysporum f.sp.dioscorea)侵染引起的一类植物真菌病害。
In order to understand the types of Dioscorea stem rot disease in Hainan. The pathogens were identified on the basis of pathogenicity testing in vitro as well as morphological characters and molecular identification. Eighty-nine diseased Dioscorea samples were collected from four villages of Dioscorea production area in Lingao and Haikou of Hainan. Twenty-eight isolates of possible pathogenic fungi were obtained from stems. Bases on the pathogenicity experiment, the results showed that lp3-1, b16-5, lp2-5 and bd6-4 were the pathogens of Dioscorea stem rot disease. The morphological identification showed that lp3-1, b16-5, lp2-5 and bd6-4 were Fusarium sp.. Then, universal primers of fungal 18S rRNA gene, specific primers of Fusarium, specific primers of Fusarium verticillioides were used to amplify related fragment, respectively. The molecular identification obtained the same results with morphology identification. After the analysis of sequences, it confirmed that Dioscorea stem rot disease was a kind of plant disease, which caused by Fusarium oxysporum f. sp. dioscorea.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第6期761-766,共6页
Genomics and Applied Biology
基金
海南省科学事业费项目(12-214001-0004)
十二五国家科技支撑计划(2012BAD19B06)共同资助
关键词
薯蓣茎腐病
鉴定
镰刀菌
系统发育分析
Dioscorea stem rot disease, Identification, Fusarium, Phylogenetic analysis
