摘要
用琼脂扩散沉淀反应检测了大肠杆菌菌毛抗原。结果表明 ,用巴比妥钠 -HCl缓冲液制备的琼脂平板 ,在菌毛孔与抗菌毛血清孔之间出现一条明显的沉淀线 ,而用 80 g/L的NaCl配制的琼脂板在菌毛孔与抗菌毛血清孔之间则无沉淀线。由此提示 ,用巴比妥钠
Avian Escherichia coli pili antigen was detected with agar gel difffusion precipitin test.There was an obvious precipitin band beween pili and anti pili serum hole when using agar plate made by barbital sodium-HCl buffer,but no band in agar plate made by 80 g/L NaCl solution was observed.This showed that agar plate made by barbital sodium-HCl buffer could be used to detect bacterial pili antigen.
出处
《湖北农学院学报》
2000年第4期343-344,共2页
Journal of Hubei Agricultural College
基金
湖北省教委资助项目 !(98A0 2 3)
关键词
鸡大肠杆菌
菌毛抗原
琼脂扩散沉淀反应
检测
Avian Escherichia coli
pili antigen
agar gel difffusion precipitin test
