摘要
猪屠宰后 ,迅速采取其皮下脂肪在 37℃下的膜提取液中的匀浆 ,于 37℃温箱中孵育 30 min后 ,用差速离心法和高速离心法提取脂肪细胞膜蛋白 ,并作 SDS- PAGE电泳分析脂肪细胞膜蛋白与其它组织细胞膜蛋白的差异。从电泳图上看出 ,脂肪细胞有很多特异膜蛋白 ,也有一些与其它组织细胞膜蛋白相似的条带。膜蛋白与佐剂充分乳化后主动免疫 3只山羊 ,免疫后 70 d取血清 ,用 EL ISA检测抗体效价为 1:6 40 0。同样 ,用 EL ISA测定抗体与其它组织细胞膜的交叉反应 ,结果显示 :抗猪脂肪细胞膜抗体与其它组织细胞膜有交叉反应 ,但反应性不高。
The adipose tissue extracted from crossbred pigs(50～80 kg)was homogenized in 37℃ membrane extractant.After that the homogenate was incubated at 37℃ for 30 minutes.Then the membrane protein of pig adipocyte was normally obtained by centrifugation for several times.Comparative analyses of membrane protein profiles between adipocyte and other tissue cell proteins was conducted by SDS-PAGE.It showed that adipcoyte possessed many specific protein profiles and some ones were similar to other tissue cells.The goat antisera against pig adipocyte membrane protein was produced by multipoint subcutaneous injection of membrane antigen in Freunds adjuvant.Goat antisera were obtained 70 days after the first booster injection and the specific antibodies titer reached 1∶6 400 detected by ELISA.Low cross-reactivity of the antisera to other pig tissues could be detected.
出处
《江苏农业学报》
CSCD
北大核心
2000年第2期97-101,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自然科学基金资助项目! (BK95 0 5 930 2 )
关键词
猪脂肪细胞
膜蛋白抗体
免疫
山羊
ELISA鉴定
adipocyte of pig
antiserum to membrane protein
immunization
goat
appraisal by ELISA
