摘要
目的使用血清学方法和PCR方法对北京地区实验小鼠多瘤病毒（POLY）感染情况进行初步调查和评估分析。方法对2011-2012年度我中心收检的不同实验动物生产设施的SPF级、清洁级小鼠血清130份、不同实验动物使用机构实验小鼠血清67份,共197份样品进行POLY病毒血清学检测;根据多瘤病毒保守序列设计引物,应用PCR方法检测不同动物生产设施的SPF级、清洁级小鼠的80份肠内容物样品是否存在POLY病毒。结果抽检的2011-2012年度实验动物生产设施的SPF级、清洁级小鼠血清130份未检出POLY病毒抗体;实验动物使用机构实验小鼠血清样品检出POLY病毒抗体7/67阳性。使用的PCR方法检测实验动物生产设施不同级别的80份小鼠肠内容物未检出POLY病毒核酸。结论北京地区实验动物饲养机构的实验动物基本排除POLY病毒的潜在污染,实验动物使用机构中实验用动物仍然存POLY病毒的潜在污染。实验动物生产设施对各级别实验动物应每年度至少进行一次全项病毒检测,以便更客观的了解实验动物质量;实验动物使用机构应对如何加强实验中动物的质量控制问题加以关注。
Objective To evaluate the prevalence of mouse polyoma virus infection in laboratory mice in Beijing area by PCR and serological tests. Methods Immunofluorescence assay was used to test the sera of 197 mice selected from the samples submitted to our monitoring center during 2011-2012. Among the 197 samples, 130 sera were from some breeding facilities, and 67 sera were from some user facilities. A poly virus specific primer pair was used in PCR to detect poly virus from intestinal contents of 80 mice from breeding facilities. Results The 130 sera from breeding facilities were negative for polyoma virus. There were 7/67 poly virus-positive samples from the user facilities. A 272 bp polyoma virus-specific amplification products can be detected in experimentally infected mice. No specific amplification products were detected in the 80 samples from breeding facilities. Conclusions Poly virus contaminations are not detected by the current serological and PCR assays in the breeding facilities during the past 2 years. The prevalence of mouse polyoma virus in user facilities should not be neglected. An annual surveillance including poly virus is necessary not only in breeding facilities, but also in user facilities.
出处
《中国比较医学杂志》
CAS
2013年第12期40-43,共4页
Chinese Journal of Comparative Medicine
基金
中央级公益性科研院所基本科研业务费专项基金项目(DWS201303)
关键词
小鼠多瘤病毒
血清学
PCR
潜在污染
Polyoma virus
Serological test
PCR
Potential contamination
