摘要
目的观察辛伐他汀体内给药对尾悬吊大鼠骨量和骨髓基质干细胞增殖﹑分化的影响。方法 18只9周龄雄性SD大鼠被随机分成3组,每组6只:第一组(G1),正常对照组,每天蒸馏水灌胃;第二组(G2),尾悬吊组,每天蒸馏水灌胃;第三组(G3),尾悬吊大鼠每天20 mg/kg辛伐他汀灌胃。实验持续3周,所有大鼠在最后一次灌胃的第二天被处死,取大鼠右侧股骨用双能X线骨密度仪测量骨密度。取大鼠左侧股骨和胫骨骨髓细胞向成骨细胞定向培养,并作如下检测:碱性磷酸酶活性和von Kossa染色分别在细胞培养第16天和25天检测。在细胞培养第21天,采用Real-time RT-PCR检测BMP-2、RANKL mRNA的表达。结果尾悬吊组大鼠骨量低于对照组。全长骨密度(tBMD)及远端骨密度(dBMD)G1组显著高于G2、G3组,近端骨密度(pBMD)G1组显著高于G2组,但与G3组没有区别,G3组高于G2组但没有显著差别。细胞外基质矿化能力(von Kossa染色)、ALP比活性以及RANKL、BMP-2 mRNA水平各组间均没有显著差别。结论尾悬吊3周可致大鼠骨骨质疏松,辛伐他汀体内给药可部分阻止股骨近端骨量丢失,但不能显著促进骨髓基质干细胞向成骨细胞分化。
Objective To investigate the effect of simvastatin on bone mass in vivo and the proliferation and differentiation of bone marrow stromal cells （BMSCs） in tail-suspended rats. Methods Eighteen 9-week male Sprague-Dawley rats were randomly divided into 3 groups, and each group had 6 rats. Rats in G1, the control group, were given a gavage of normal saline every day. Rats in G2, the tail-suspended group, were also given a gavage of normal saline every day. Rats in G3 were tail-suspended and given a gavage of 20 mg/kg simvastatin per day. The experiment lasted for 3 weeks, and all the rats were sacrificed one day after the last gavage. The right femurs were removed for the measurement of bone mineral density （BMD） using dual-energy X-ray absorptiometry. BMSCs were collected from the left femur and the tibiae and cultured for differentiation into osteoblasts. Detection of alkaline phosphatase （ALP） activity and von Kossa staining were performed at the 16＇h and 25~ day, respectively. Real-time RT-PCR was performed to detect the mRNA expression of BMP-2 and RANKL at the 21＂ day. Results The bone mass of rats in tail-suspended group was lower than that in control group. The total BMD （tBMD） and distal BMD （dBMD） in G1 was significantly higher than that in G2 and G3. And the proximal BMD （pBMD） in GI was significantly higher than that in G2, but it had no significant difference with that in G3. The pBMD in G3 was higher than that in G2, but no significant difference was observed. Von Kossa staining, ALP activity, and the mRNA expression of BMP-2 and RANKL showed no significant difference among groups. Conclusion Three-week tail-suspension can lead to osteoporosis in SD rats. Simvastatin can partially prevent the bone loss in the proximal femur in vivo, but cannot significantly promote the BMSCs differentiation into osteoblasts.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第12期1228-1231,共4页
Chinese Journal of Osteoporosis
基金
河北省自然科学基金资助项目(C2006000580)
关键词
辛伐他汀
尾悬吊
骨密度
骨髓基质细胞
成骨细胞
Simvastatin
Tail-suspension
Bone mineral density
Bone marrow stromal cells
Osteoblast
