摘要
目的分析干化学法和湿化学法测定血糖的相关程度,探讨两种方法能否共用同一参考区间,保持检测结果的一致性。方法依据美国临床实验室标准化协会的EP9-A2文件,以ADVIA2400生化仪组成的检测系统作为比较方法,以VITROS FS 5.1生化仪组成的检测系统作为实验方法,分析两种方法之间的相关性,换算所得直线回归方程的斜率、截距作为干化学仪的校正参数,再重复测定血糖,分析参数改变前后两种方法的相关性和临床可接受性。结果参数改变前,两种方法检测血糖相关性很好,相关系数r2=0.999 5,但是不同医学决定水平的系统误差均大于允许偏差(EA)的1/2,校正参数后,两种方法检测血糖r2=0.999 7,SE均小于EA的1/2。结论干化学法和湿化学法测定血糖浓度相关性良好,通过校正参数后可共用同一参考区间,其检测结果临床可接受。
Objective To study the correlation of blood glucose results by dry chemical and liquid chemical a-nalysis ,explore whether these two methods can share a reference interval ,while keep the result consistency . Methods According to EP9-A document request of National Committee for Clinical Laboratory of Standards ,the comparative method（X） was ADVIA2400 ,VITROS FS 5 .1 examination method（Y） ,analyze the correlation between the two methods .Detected the blood sugar level again by these two methods after the dry chemical analysis parame-ters were changed .Results Before changes in the parameters ,the blood sugar level by the two methods correlated well（r2 =0 .999 5） ,however the expected bias（SE） of the different Medicine decide level was greater than the allowa-ble deviation （EA/2 ） .After parameters calibration ,these two methods of blood sugar correlated well too （ r2 =0 .999 7） ,SE was less than EA/2 .Conclusion Dry chemical and liquid chemical analysis have good correlation in blood sugar concentration ,they can share the same reference interval after parameters calibration ,which can be re-ceived in clinic .
出处
《检验医学与临床》
CAS
2013年第24期3318-3319,共2页
Laboratory Medicine and Clinic
关键词
干化学法
参考区间
血糖
相关分析
湿化学法
dry chemical analysis
reference interval
blood sugar
correlation analysis
liquid chemi-cal method
