摘要
目的探讨IFN-γ对胆囊癌小鼠模型中肿瘤内IL-10和单核-巨噬细胞的影响。方法20只BALB／C裸鼠通过皮下接种人胆囊癌细胞株GBC—SD构建胆囊癌小鼠模型，按照随机数字表法分为IFN-γ组和对照组（每组各10只）。IFN．叮组小鼠在肿瘤内注剃、鼠重组IFN-γ 0．1mL（1×10^5kU／L，生理盐水溶解），对照组注射同等剂量的生理盐水，通过ELISA法检测肿瘤中鼠源性IL-10的表达情况，通过免疫组织化学染色法计数CDl4’细胞（单核-巨噬细胞）、CD64^＋细胞（M1型巨噬细胞）和CD206’细胞（M2型巨噬细胞），并以Student’st检验对所得数据进行分析。结果接种肿瘤细胞1周后，裸鼠皮下全部成瘤，固定于左前肢腋下。IFN-γ组存活9只，对照组存活7只。IFN-γ组裸鼠肿瘤质量为（518±138）mg，对照组为（669±128）mg；IFN．1组肿瘤体积为（456±172）mm。，对照组为（505±146）mm。，两组裸鼠肿瘤质量和体积比较，差异无统计学意义（t=2．240，1．503，P〉0．05）。IFN-γ组裸鼠肿瘤中鼠源性IL-10浓度为（58±16）μg／g，显著低于对照组的（102±45）“g／g，两组比较，差异有统计学意义（t=2．796，P〈0．05）。IFN-γ组中单核-巨噬细胞计数为814-16，显著高于对照组的50-e21；M1型巨噬细胞计数为66±12，显著高于对照组的94-4；M2型巨噬细胞计数为154-4，显著低于对照组的40±14，两组细胞计数比较，差异均有统计学意义（t=3．214，13．127，6．914，P〈0．05）。结论IFN-γ能够降低肿瘤微环境中的IL-10浓度。IFN-γ能够诱导单核-巨噬细胞浸润至胆囊癌细胞问质中，主要分化为M1型巨噬细胞。
Objective To investigate the effects of interferon-γ （IFN-γ） on interleukin- 10 （ IL- 10） and mononuelear macrophages in a mouse model of gallb]adder cancer. Methods Mouse models of gallbladder cancer were constructed by inoculating the human gallbladder cancer cell line GBC-SD subcutaneously in 20 BALB/C mice, and then all the mice were randomly divided into the IFN-γ group and the control group （ 10 mice in each group）. Murine recombinant IFN-γ （0. 1 mL, 1 × 10^5 kU/L, diluted with normal saline） was injected into the tumors in the IFN-γgroup, and normal saline was injected into the tumors in the control group. The expression of IL-10 was detected by ELISA, and the numbers of CD14 ± cells （mononuclear macrophages） , CD64 ± cells （M1 macrophages） and CD206 ± cells （M2 maerophages） were counted by the immunohistochemistry. All data were analyzed using the Student＇s t test. Results The mouse models of gallbladder cancer were successfully constructed 1 week later. Nine mice survived in the IFN-γ group, and 7 mice survived in the control group. The tumor weight was （518 ± 138）mg in the IFN-γgroup and （669± 128）mg in the control group, with a significant differ- ence between the 2 groups （t =2. 240, P〉0.05）. The volume of the tumor was （456 ± 172）ram3 in the IFN-~ group and （505 ± 146）ram3 in the control group, with no significant difference between the 2 groups （t = 1. 503, P 〉 0. 05 ）. The concentration of IL-IO was （ 58± 16） Ixg/g in the IFN-＇y group, which was significantly lower than （ 102 ± 45 ） μg/g in the control group（ t = 2. 796, P 〈 0.05 ）. The number of mononuclear macrophages was 81 ± 16 in the IFN-γgroup, which was significantly greater than 50 ± 21 in the control group; the number of M1 macro- phages was 66 ± 12 in the IFN-＇y group, which was significantly greater than 9 ± 4 in the control group; the number of M2 macrophages was 15 ± 4 in the IFN-γgroup, which was significantly lower than 40 ± 14 in th
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2014年第1期51-54,共4页
Chinese Journal of Digestive Surgery
基金
辽宁省科技计划课题（2009225010-19）
