摘要
目的了解幼兔胎粪吸入后肺组织中血栓素Ｂ２（ＴＸＢ２）和６－酮前列环素１α（６—ｋｅｔｏ－ＰＧＦ１α）的动态变化。方法将动物分为轻度胎粪吸入组（１６ ｈ，２４ ｈ，４８ ｈ，７２ ｈ）、重度胎粪吸入组和对照组，通过气管内灌入胎粪０．６ ｍｌ／ｋｇ和 ４ ｍｌ／ｋｇ建立轻、重度幼兔胎粪吸入模型；应用右心室穿刺法经压力传感器与日本光电公司生产ＲＭ－６０００型多道生理记录仪相连，测定右心室收缩压；应用放射免疫法检测肺组织中ＴＷ和６－ｋｅｔｏ－ＰＧＦ１α含量。结果轻度胎粪吸入组右心室收缩压从胎粪吸入后 １６ ｈ升高（２ ５７± ０． １０ ｋＰａ）， ２４ ｈ达高峰（３． ５７± ０．１４ｋＰａ），７２ ｈ恢复正常（１．８９± ０． １４ ｋＰａ）。重度胎粪吸入组右心室收缩压（４． ３６± ０． １４ ｋＰａ）明显高于轻度胎粪吸入组和对照组（１．８５±０．０５ ｋＰａ）；轻度幼兔胎粪吸入１６ ｈ肺组织ＴＸＢ２（１５３．８０±１５．３７ ｐｇ／ｍｌ）和６－ｋｅｔｏ－ＰＧＦ１α（１１７．４０±２２．８８ｐｇ／ｍｌ）水平开始增高，２４ｈ达高峰（３６．９００±２８．８０ｐｇ／ｍｌ；２０７．２０±２８．５９ｐｇ／ｍｌ），至７２ｈ开始降低（ １０３．２０± ?
Objective To study the changes of TXB2 and 6 - keto - PGF1α in the, lung of immature rabbits with meconium aspiration. Methods We established mild and severe immature rabbit models of meconium aspiration by endotracheal intubation imbuing meconium 0. 6 ml/kg and 4 ml/kg. We measured the right ventricular pressure by right ventricular puncture. Levels of TXB2 and 6 - keto - PGF1α in the lung were measured by radioimmunoassay. Results In the mild group, the right ventricular pressure began to increase at 16 hours (2. 57 ± 0. 10 kPa), peaked at 24 hours (3. 57 ± 0. 14 kpa) and returned to the normal level by 72 hours (1. 89 ± 0. 04 kPa). The pressure of the severe group (4. 36 ± 0. 14 kpa)was significantly higher than that of the control (1. 85 ± 0. 05 kPa) and mild groups. Levels of TXB2 and 6 - keto - PGF1α. in the lung of the mild group increased significantly compared with the control group (76. 40 ± 16. 89 pg/ml; 56. 10 ± 16. 46 pg/ml), began to increase at 16 hours (153. 80 ± 15. 37 pg/ml, 117. 40 ± 22. 88 pg/ml),. peaked at 24 hours (369. 00 ± 28. 80 pg/ml; 207. 20 ± 28. 59 pg/ml) and recovered by 72 hours (103. 20 ± 11. 95 pg/ ml, 83. 62 ± 8. 76 pg/ml). The changes of the severe group (500. 60 ± 24. 58 pg/ml; 300. 00 ± 20. 31 pg/ml) were significantly different from those in the control and mild groups. The level of TXB2 and 6 - keto - PGF1. were correlated with the right ventricular pressure (r = 0. 95: 0. 96. P <0. 01). Conclusions TXB2 and 6 - keto -PGF1α in the lung significantly increase following the severity of meconium aspiration, indicating that TXB2 and 6 - keto - PGF1. influence the development of lung injury of meconium aspiration.
出处
《中国当代儿科杂志》
CAS
CSCD
2000年第6期395-397,共3页
Chinese Journal of Contemporary Pediatrics
