摘要
目的探讨瑞舒伐他汀钙对尼古丁诱导人脐静脉内皮细胞（HUVEC）基质金属蛋白酶2（MMP2）表达的影响。方法取健康剖腹产孕妇的HUVEC进行培养，选择2～4代细胞进行实验（n=5）。分别采用不同浓度（0、1、10、100／μmol／L）尼古丁进行24h处理以及采用100μmol／L尼古丁进行不同时间（0、6、12、24、48h）处理，处理后细胞进行半定量RT-PCR，Western blot检测MMP-2mRNA及蛋白的表达（n=5）；内皮细胞首先用系列浓度（2，4，7，10／μg／L）瑞舒伐他汀钙进行预孵育12h，然后对药物干预后细胞进行100μmol／L尼古丁处理24h（n=50）。结果尼古丁呈浓度及时间依赖性诱导HuVEc中MMP-2表达：MMP2表达随尼古丁浓度增加而升高；尼古丁诱导MMP2表达随时间延长而增加。瑞舒伐他汀钙可浓度依赖性地抑制尼古丁诱导的MMP-2的表达[mRNA水平：2、4、7、10μg／L抑制率分别为8．6％、42．5％、66．9％、80．3％，均P〈0．05；蛋白水平：2、4、7、10μg／L抑制率分别为10．6％、37．6％、74．1％、88．2％，均P〈0．051。结论尼古丁是诱导HUVEC中MMP表达的重要因素；瑞舒伐他汀钙能够抑制该进程，且具有剂量依赖性。
Objective To explore the effect of rosuvastatin on the expression of matrix metalloproteinase-2（MMP2 ） in human umbilical vein endothelial cells （HUVEC） induced by nicotine. Methods HUVEC were isolated from umbilical cords of healthy caesarean women and cells at passages 2-4 were used in this study （n= 5）. Cells were treated with different concentration of nicotine （0, 1, 10, 100 μmol/L） for 24 h or treatd with 100 μmol/L for different time （0, 6, 12, 24, 48 h）, and then the expression of MMP-2 mRNA and protein in cells of the two groups were detected using semiquantitative real time PCR and Western blot （ n=5）. Cells were incubated in series concentration rosuvastatin calcium （2, 4, 7, 10 μg/L） for 12 h, and then the incubated cells were treated with 100 μmol/L nicotine for 24 h after pharmacologic interventions （n 5）. Results HUVEC MMP 2 expression was induced by nicotine in a concentration-dependent and time-dependent manner. The expression of MMP-2 induced by nicotine was inhibited by rosuvastatin calcium in a concentration-dependent [on mRNA level： the inhibition rates of 2, 4, 7, 10 μg/L were 8.6%, 42.5%, 66.9% and 80.3% respectively, all P〈0.05; on protein level： the inhibition rates of 2, 4, 7, 10μg/L were 10. 6%, 37.6%, 74.1%, 88.2% respectively, all P.〈0. 05]. Conclusion Nicotine is a major factor which can induce the expression of MMP-2 in HUVEC, which is a dangerous pathogenic factor in smoking. Rosuvastatin calcium can inhibit the process in a dose dependent manner.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2013年第11期1066-1070,共5页
Chinese Journal of Hypertension
关键词
尼古丁
脐静脉内皮细胞
基质金属蛋白酶
瑞舒伐他汀钙
Nicotine
Human umbilical vein endothelial cells
Matrix metalloproteinase
Rosuvastatin calcium
