摘要
目的探讨七氟醚预处理和后处理对大鼠肾缺血-再灌注损伤的影响。方法SD雄性大鼠36只，体重220-240g，随机均分为四组：假手术组（A组）、缺血-再灌注组（B组）、七氟醚预处理组（C组）和七氟醚后处理组（D组）。采用结扎右侧肾动脉，夹闭左侧肾动脉45min、再灌注24h的方法制备肾缺血-再灌注模型。A组仅切开腹腔，游离两侧肾蒂，但不阻断任何动脉；C组于制备模型前吸入七氟醚45min，浓度维持在1MAC（2．5％）；D组在氯胺酮100mg／kg麻醉开放动脉后吸入1MAC七氟醚并维持45min，其余步骤同其他各组。缺血-再灌注后24h分别测定血浆及肾组织超氧物歧化酶（SOD）、丙二醛（MDA）和髓过氧化物酶（MPO）的含量。对肾小管损害进行Paller评分。结果B．C、D组血清与肾组织中SOD含量明显低于，MDA、MPO明显高于A组（P〈0．01）；C、D组血清与肾组织SOD含量明显高于，MDA、MPO含量明显低于B组（P〈0．05）。B、C及D组的肾小管Paller评分明显高于A组，且C、D组明显低于B组（P〈0．05）。结论七氟醚预处理和后处理均可通过氧自由基介导作用减轻肾脏损伤，两者对改善肾缺血-再灌注损伤的程度无明显差别。
Objective To investigate the two methods （namely pre and post-conditioning） of sevoflurane on renal ischemia reperfusion injury. Methods Thirty-six male SD rats, weighted 220-240 g were randomly divided into four groups （n= 9）： sham operation group （group A）, ischemia- reperfusion group （group B）, sevoflurane pre-conditioning group （group C） and sevoflurane post- conditioning group （group D）. Renal ischemia and reperfusion model was prepared by ligating the right renal artery and occluding the left renal artery for 45 min followed by repeffusion for 24 h （group B）. In groups C and D, 1 MAC of sevoflurane was inhaled for 45 mins before model preparation or after declamping the left renal artery, respectively. Twenty-four hours after ischemia and reperfusion, SOD, MDA, and MPO levels in the blood and renal tissue were measured. The pathological changes of kidney tissue were investigated and renal tubular damages were analyzed with Paller method. Results Compared with group A, the levels of SOD in the plasma and renal tissues decreased significantly, and MDA and MPO levels increased significantly （P〈0. 01） in group B. Compared with group B, the levels of SOD in the plasma and renal tissues was significantly higher, and MDA and MPO levels were significantly lower in groups C and D （P〈0.05）. No significant differences in SOD, MDA and MPO levels were found between groups C and D. For the Tubular Paller＇s score, the scores in groups B, C and D were significantly higher than that in group A （P〈 0.05）. The scores in groups C and D were significantly lower than that in goup B. No differences were observed between groups C and D. Conclusion Sevoflurane preconditioning and post- conditioning can both reduce renal damage through oxygen free radical-mediated effects. The protective effects are similar between these two methods.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2013年第11期1114-1116,共3页
Journal of Clinical Anesthesiology
关键词
七氟醚
活性氧
肾缺血-再灌注损伤
预处理
后处理
Sevoflurane
Reactive oxygen species
Renal ischemia and reperfusiom Pre-conditioning
Post-conditioning
