摘要
目的评价七氟醚预处理对大鼠肾缺血再灌注损伤的影响。方法雄性SD大鼠24只，体重250～300g，采用随机数字表法，将大鼠随机分为3组（n=8）：假手术组（S组）、肾缺血再灌注组（I／R组）和七氟醚预处理组（SP组）。I／R组和SP组采用切除右肾然后夹闭左侧肾动脉45min再开放的方法制备肾缺血再灌注模型。SP组吸入2．2％七氟醚1h，停止吸入后10min时进行肾缺血。于再灌注2h时采集静脉血样，测定血清肌酐（Cr）、尿素氮（BUN）和胱抑素C（CysC）的浓度，取肾组织，光镜下及透射电镜下观察病理学结果，并根据肾小管病变程度进行Paller评分。结果与s组比较，I／R组血清cr和BUN浓度差异无统计学意义（P〉0．05），血清CysC浓度和Paller评分明显升高（P〈0．05）；与I／R组比较，SP组血清CysC浓度和Paller评分明显降低（P〈0．05）。sP组肾组织损伤程度轻于I／R组。结论七氟醚预处理可减轻大鼠肾缺血再灌注损伤。
Objective To investigate the effects of sevoflurane preconditioning on renal ischemia-reperfusion （I/R） injury in rats. Methods Twenty-four adult male SD rats weighing 250-300 g were randomly divided into 3 groups （ n = 8 each） ： sham operation group （group S） ; I/R group; sevoflurane preconditioning group （group SP）. After the rats underwent right nephrectomy, renal I/R was produced by occlusion of left renal artery for 45 min followed by reperfusion in I/R and SP groups. In group SP, the rats inhaled 2.2% sevoflurane for 1 h, then the inhalation was stopped and renal ischemia was performed 10 min later. Venous blood samples were collected at 2 h of reperfusion to determine the concentrations of serum creatinine （ Cr）, urea nitrogen （BUN）, cystatin C （ Cys C）. The renal tissues were obtained for microscopic examination, and Paller＇s score was recorded. Results Compared with group S, there was no significant difference in the serum Cr and BUN concentrations （ P 〉 0.05）, while the serum Cys C concentration and Paller＇ s score for acute renal tubular injury were significantly increased in group I/R （P 〈 0.05）. The serum Cys C concentration and Paller＇ s score were significantly lower in group SP than in group I/R （P 〈 0.05）. I/R-induced renal injury was significantly reduced in group SP compared with group I/R. Conclusion Preconditioning with sevoflurane can provide significant protection against renal I/R injury.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第3期368-371,共4页
Chinese Journal of Anesthesiology
关键词
缺血预处理
再灌注损伤
肾
七氟醚
Ischemic preconditioning
Reperfusion injury
Kidney
Sevoflurane
