摘要
目的:探讨异丙酚对肾缺血-再灌注后肺损伤是否具有保护作用,以及在不同时间点给予异丙酚,其保护作用是否有差异。方法:Wistar大鼠48只,随机分为假手术对照组(C组)、肾缺血-再灌注组(R组),以及异丙酚用药组[分别在缺血前1h(P1组)、缺血即刻(P2组)、再灌注即刻(P3组)和再灌注后1h(P4组)给予异丙酚],共6组,异丙酚输注速度为20mg/(kg·h),共3h。制作肾缺血-再灌注模型。实验结束即刻取动物血样及肺组织,测定超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及肺组织形态学变化。结果:与C组相比,R组红细胞及肺组织的SOD活性明显降低(均P<0.01),血清及肺组织的MDA含量明显升高(均P<0.01),光镜下,R组肺组织结构明显受损;与R组相比,各用药组除P4组外,均有所改善;各用药组间变化也有差异。结论:肾缺血-再灌注会引起肺损伤;异丙酚对肾缺血-再灌注所造成的肺损伤有良好的保护作用;给药时间点不同,对异丙酚的保护作用有影响。
Objective To study the effects of propofol on lung injury induced by renal ischemia-reperfusion in rats. Methods Total 48 Wistar rats were randomly divided into 6 groups, including Group C （sham-operated animals）, Group R （renal ischemia-reperfusion）, Group P1 （administration of propofol at 1 hour before ischemia）, Group P2 （at the moment of ischemia）, Group P3 （at the moment of reperfusion）, Group P4 （at 1 hour after reperfusion）. At the end of the reperfusion period, blood samples and lung tissues were collected. SOD activity, MDA concentration and morphologic changes of the lungs were examined. Results Compared with Group C, SOD activity decreased significantly and MDA concentration increased significantly in Group R（P 〈 0.01 ）. Light microscopic findings show the pulmonary structure was badly injuried in group R. Compared with Group R, the injured severity was obviously relieved in the groups which received propofol excluding Group P4. The changes were different in the groups which received propofol. Conclusions Renal ischemia-reperfusion can lead to injury of lung. Poropofol can effectively protect pulmonary injury induced by renal ischemia-reperfusion. Different time in which propofol was administrated can influent on the protection of propofol.
出处
《实用医学杂志》
CAS
2007年第11期1608-1610,共3页
The Journal of Practical Medicine
关键词
再灌注损伤
肾
二异丙酚
大鼠
肺损伤
Reperfusion injury Kidney Propofol Rats Lung injury
