摘要
目的监测二级医院耐甲氧西林金黄色葡萄球菌(MRSA)及耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)的流行及耐药现状,为临床合理应用抗菌药物提供依据,减缓葡萄球菌属多药耐药菌株的产生。方法对医院从2010年7月-2012年6月的感染标本中分离的180株葡萄球菌属用HX-21型微生物分析仪进行细菌鉴定和药敏分析,纸片扩散法(K-B法)对药敏试验进行补充,数据采用MicroScan Labpro2.41软件进行统计分析。结果共分离出葡萄球菌属180株,其中金黄色葡萄球菌55株占30.56%,凝固酶阴性葡萄球菌125株占69.44%;180株葡萄球菌属中108株为耐甲氧西林株占60.00%,MRSA与MRCNS分别占金黄色葡萄球菌和凝固酶阴性葡萄球菌的40.00%和76.80%;甲氧西林敏感金黄色葡萄球菌(MSSA)和甲氧西林敏感凝固酶阴性葡萄球菌(MSCNS)分别占金黄色葡萄球菌和凝固酶阴性葡萄球菌的60.00%和23.20%;MRSA与MRCNS均为多药耐药菌株,均未发现耐万古霉素、替考拉宁、利奈唑胺菌株。结论二级医院MRSA与MRCNS的流行严重,二者多药耐药菌株呈上升趋势。
OBJECTIVE To monitor the prevalence and current drug resistance condition of methicillin-resistant Staphylococcus aureus （MRSA） and methicillin-resistant coagulase negative Staphylococcus （MRCNS） in second-ary hospital, and provide basis for clinical rational application of antimicrobial drugs, so as to slow the production speed of multi-drug resistant strains in Staphylococcus. METHODS A total of 180 strains of Staphylococcus isola- ted from the infection specimens in the hospital from Jul. 2010 to Jun. 2012 were processed with HX-type 21 microbial analyzer for bacterial identification and drug sensitive analysis with the supplement of Kirby-Bauer meth-od （K-B method）. The data were statistically analyzed using MicroScan Labpro 2.41 software. RESULTS A total of 180 strains of Staphylococcus were isolated, including 55 strains of Staphylococcus aureus, accounted for 30.56% ; 125 strains of coagulase negative Staphylococcus, accounted for 69.44% ; 108 in 180 strains of Staphy- lococcus were methicillin resistant （60.0%）, MRSA and MRCNS accounted for 40.0% and 76.80% of S. aureus and coagulase negative Staphylococcus （CNS） respectively. Methicillin sensitive S. aureus （MSSA） and methicillin sensitive coagulase negative Staphylococcus （MSCNS） accounted for 60. 0% and 23.20% in the S. aureus and coagulase negative Staphylococcus respectively. MRSA and MRCNS were both multi-drug resistant strains. The vancomycin-resistant strains, teicoplanin-resistant strains and linezolid-resistant strains were not found. CONCLUSION The prevalence of MRSA and MRCNS is serious in secondary hospital, and multi-drug resistant strains shows an ascendant trend.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第23期5835-5837,共3页
Chinese Journal of Nosocomiology
基金
咸宁市科学技术研究基金项目(20130026)
关键词
耐甲氧西林葡萄球菌
流行
耐药性
Methicillin-resistant Staphylococcus aureus
Prevalence
Drug resistance
